等离子体诱导甲基丙烯酸羟乙酯在硅橡胶和聚4-甲基-1-戊烯薄膜上的接枝共聚反应改善兔角膜上皮细胞的附着和生长。
Plasma-induced graft copolymerization of HEMA onto silicone rubber and TPX film improving rabbit corneal epithelial cell attachment and growth.
作者信息
Hsiue G H, Lee S D, Wang C C, Shiue M H, Chang P C
机构信息
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, R.O.C.
出版信息
Biomaterials. 1994 Feb;15(3):163-71. doi: 10.1016/0142-9612(94)90062-0.
A poly(2-hydroxyethyl methacrylate) (pHEMA)-grafted polymer film was prepared by plasma-induced graft copolymerization onto an elastic material, silicone rubber, and a plastic material, poly(4-methyl-1-pentene) (TPX). The control, Ar plasma-treated and pHEMA-grafted silicone rubber and TPX surfaces were characterized by ESCA, FTIR-ATR, SEM and contact angle techniques. ESCA verified the respective chemical shift of control and Ar plasma-treated films. The presence of the grafted pHEMA was also verified by ESCA. The introduction of pHEMA onto a hydrophobic support provided an adequate surface for rabbit corneal epithelium cell attachment and growth. Cell attachment and growth onto these surfaces were examined by light microscopy. Cell attachment onto the control and Ar plasma-treated surfaces was negligible, while improved attachment and growth of rabbit corneal epithelium cells was demonstrated on the pHEMA-grafted polymeric surface. At 72 h, the pHEMA-grafted silicone rubber surface attached and grew more cells as compared with those on a pHEMA-grafted TPX surface. The pHEMA-grafted silicone rubber surface demonstrated a confluent cell layer after 72 h.
通过等离子体诱导接枝共聚反应，在弹性材料硅橡胶和塑料材料聚（4-甲基-1-戊烯）（TPX）上制备了聚甲基丙烯酸2-羟乙酯（pHEMA）接枝聚合物薄膜。采用光电子能谱（ESCA）、傅里叶变换红外光谱-衰减全反射（FTIR-ATR）、扫描电子显微镜（SEM）和接触角技术对未处理的、经氩等离子体处理的以及pHEMA接枝的硅橡胶和TPX表面进行了表征。ESCA证实了未处理的和经氩等离子体处理的薄膜各自的化学位移。ESCA还证实了接枝的pHEMA的存在。将pHEMA引入疏水载体上为兔角膜上皮细胞的附着和生长提供了合适的表面。通过光学显微镜检查细胞在这些表面上的附着和生长情况。细胞在未处理的和经氩等离子体处理的表面上的附着可以忽略不计，而在pHEMA接枝的聚合物表面上兔角膜上皮细胞的附着和生长得到了改善。在72小时时，与pHEMA接枝的TPX表面相比，pHEMA接枝的硅橡胶表面附着和生长的细胞更多。72小时后，pHEMA接枝的硅橡胶表面形成了汇合的细胞层。
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