含有苏云金芽孢杆菌（B.t.）和豇豆胰蛋白酶抑制剂（CpTI）基因的高抗虫转基因烟草植株。
Highly insect-resistant transgenic tobacco plants containing both B.t. and CpTI genes.
作者信息
Zhao R, Fan Y, Shi X, Wang J, Zong W
机构信息
Laboratory of Molecular Biology, Chinese Academy of Agricultural Sciences, Beijing.
出版信息
Chin J Biotechnol. 1995;11(1):1-7.
The cowpea trypsin inhibitor (CpTI) gene was synthesized according to its cDNA sequence using DNA synthesizer and confirmed by DNA sequencing. The CpTI gene and modified Bacillus thuringiensis (B.t.) delta-endotoxin gene were cotransformed to tobacco explants mediated by Agrobacterium tumefaciens. The integrations of B.t and CpTI genes were confirmed by PCR and Southern hybridization. Three kinds of transgenic plants were obtained: (1) containing CpTI gene, (2) containing B.t gene, (3) containing both CpTI and B.t genes. Bioassays showed that all these transgenic plants were toxic to the larvae of Heliothis armigera and that the tobacco plants containing both genes had enhanced toxicity to larvae by comparison with plants containing only CpTI or B.t gene.
根据豇豆胰蛋白酶抑制剂（CpTI）基因的cDNA序列，使用DNA合成仪合成该基因，并通过DNA测序进行确认。将CpTI基因和修饰的苏云金芽孢杆菌（B.t.）δ-内毒素基因通过根癌农杆菌介导共转化到烟草外植体中。通过PCR和Southern杂交确认了B.t.基因和CpTI基因的整合。获得了三种转基因植物：（1）含有CpTI基因；（2）含有B.t.基因；（3）同时含有CpTI和B.t.基因。生物测定表明，所有这些转基因植物对棉铃虫幼虫都有毒性，并且与仅含有CpTI或B.t.基因的植物相比，同时含有这两个基因的烟草植物对幼虫的毒性增强。
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