[利用针对长末端重复序列的核酶体外切割牛白血病病毒RNA的条件研究]
[Study of conditions for cleaving bovine leukemia virus RNA in vitro using ribozymes directed against long terminal repeats].
作者信息
Zelivianskiĭ S V, Karpov V A, Baurin V V, Miroshnichenko O I, Tikhonenko T I
出版信息
Mol Gen Mikrobiol Virusol. 1994 May-Jun(3):25-8.
Ribozyme has been constructed by the oligonucleotide synthesis technique that is capable of splitting bovine leukemia virus (BLV) RNA-substrate in the R-region (358th nucleotide). Ribozyme has been shown to efficiently split the RNA containing R-region of the virus. The efficiency of ribozyme function depends on conditions of the reaction reaching its maximum at 25 degrees C and pH 7.3-7.6. The efficiency of splitting is increased by multiple heating-cooling cycles of the substrate-ribozyme mixture.
核酶是通过寡核苷酸合成技术构建的，它能够切割牛白血病病毒（BLV）RNA底物的R区域（第358个核苷酸）。已证明核酶能有效切割含有该病毒R区域的RNA。核酶的功能效率取决于反应条件，在25摄氏度和pH值7.3 - 7.6时达到最大值。底物 - 核酶混合物的多次加热 - 冷却循环可提高切割效率。
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