Mourad W A, Vallières E
Department of Pathology, University of Alberta Hospitals, Edmonton, Canada.
In Vivo. 1995 Mar-Apr;9(2):149-54.
Ex-vivo needle aspiration (xvFNA) has been rarely used to obtain viable tumor cells. It has been occasionally employed for short-term cultures. Xenografting of lung carcinoma in athymic nude mice provides a good animal model for the study of this neoplasm. Successful engraftment using conventional methods has been disappointing) low (d 40%). Enzymatic digestion of the tumor fragments to obtain cell suspension lowers viability. We postulated that xvFNA might provide readily available tumor cell suspensions for xenografting lung carcinoma and that it would provide a higher success rate of engraftment than the conventional techniques. We aseptically performed xnFNA in 35 cases of freshly resected non-small cell carcinoma of the lung. These included 15 adenocarcinomas, 17 squamous carcinomas and 3 undifferentiated non-small cell carcinoma (UNSCC). Tumor cell suspensions were injected subcutaneously in athymic nude mice. Tumor necrosis in the aspirates ranged from 20-90% (median 60%). Gross evidence of engraftment was seen in 30 of 35 cases (85.7%) 1-19 weeks postimplantation (median 2 weeks). This was seen in UNSCC (3/3), squamous carcinomas (13/17) and adenocarcinomas (14/15). Xenograft sizes ranged from 5-34 mm (median 19 mm). They showed similar morphology to the primary tumors. Ex-vivo FNA used for harvesting lung carcinoma cells and their xenografting is an effective method for obtaining viable material for studying this neoplasm.
体外针吸活检（xvFNA）很少用于获取活的肿瘤细胞。它偶尔用于短期培养。将肺癌移植到无胸腺裸鼠体内为研究这种肿瘤提供了一个良好的动物模型。使用传统方法成功移植的情况令人失望（成功率低，仅40%）。对肿瘤碎片进行酶消化以获得细胞悬液会降低细胞活力。我们推测，xvFNA可能为肺癌异种移植提供易于获得的肿瘤细胞悬液，并且其移植成功率会高于传统技术。我们对35例新鲜切除的非小细胞肺癌患者进行了无菌xvFNA操作。其中包括15例腺癌、17例鳞癌和3例未分化非小细胞癌（UNSCC）。将肿瘤细胞悬液皮下注射到无胸腺裸鼠体内。吸出物中的肿瘤坏死率为20% - 90%（中位数为60%）。在植入后1 - 19周（中位数为2周），35例中有30例（85.7%）出现了明显的移植证据。在UNSCC（3/3）、鳞癌（13/17）和腺癌（14/15）中均观察到这种情况。异种移植瘤大小为5 - 34毫米（中位数为19毫米）。它们显示出与原发肿瘤相似的形态。用于获取肺癌细胞及其异种移植的体外FNA是获取用于研究这种肿瘤的活材料的有效方法。
