北朝鲜杨苯丙氨酸解氨酶基因家族的结构表征及mRNA水平测定
Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis.
作者信息
Osakabe Y, Osakabe K, Kawai S, Katayama Y, Morohoshi N
机构信息
Laboratory of Wood Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
出版信息
Plant Mol Biol. 1995 Sep;28(6):1133-41. doi: 10.1007/BF00032674.
We isolated two new PAL genes, palg2b and palg4, from Populus kitakamiensis, palg2a and palg2b are clustered and palg2b encodes a polypeptide of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and palg2b have several elements conserved among many phenylpropanoid biosynthetic genes. We measured the mRNA levels of the four PAL genes by S1 mapping using total RNA from stem tissues developing secondary xylem. Results showed that the transcript level of palg2b was higher than that of the other PAL genes.
我们从日本山杨中分离出两个新的PAL基因，即palg2b和palg4，palg2a和palg2b成簇排列，palg2b编码一个由710个氨基酸组成的多肽。palg2b编码区的核苷酸序列与palg2a的核苷酸序列一致性为94.6%。palg1、palg2a和palg2b的启动子区域有几个在许多苯丙烷生物合成基因中保守的元件。我们使用来自发育次生木质部的茎组织的总RNA，通过S1作图法测量了这四个PAL基因的mRNA水平。结果表明，palg2b的转录水平高于其他PAL基因。
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