Matsufuji H, Matsui T, Ohshige S, Kawasaki T, Osajima K, Osajima Y
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Biosci Biotechnol Biochem. 1995 Aug;59(8):1398-401. doi: 10.1271/bbb.59.1398.
When angiotensin fragments, Val-Tyr and Angiotensin III (ANG III), with potent ACE inhibitory activity were intravenously administered to spontaneously hypertensive rat (SHR), a significant reduction of diastolic blood pressure was observed. After incubation of ANG III with SHR plasma, four fragments with ACE inhibitory activity, Val-Tyr (ANG (3-4)) (IC50 = 26.0 microM), Ile-His-Pro-Phe (ANG (5-8)) (11.6 microM), Tyr-Ile-His-Pro-Phe (ANG (4-8)) (457.5 microM), and Val-Tyr-Ile-His-Pro-Phe (ANG (3-8)) (6.55 microM), were confirmed to generate in SHR plasma. Compared the metabolic behavior of ANG II in SHR plasma with that in normotensive Wistar plasma, the initial degradation rate (3.07 nmol/ml/min) in Wistar plasma was about 2-fold higher than that in the SHR one (1.75 nmol/ml/min).
当具有强大的血管紧张素转换酶（ACE）抑制活性的血管紧张素片段Val-Tyr和血管紧张素III（ANG III）静脉注射给自发性高血压大鼠（SHR）时，观察到舒张压显著降低。将ANG III与SHR血浆一起孵育后，在SHR血浆中确认产生了四个具有ACE抑制活性的片段，即Val-Tyr（ANG(3-4)）（IC50 = 26.0微摩尔）、Ile-His-Pro-Phe（ANG(5-8)）（11.6微摩尔）、Tyr-Ile-His-Pro-Phe（ANG(4-8)）（457.5微摩尔）和Val-Tyr-Ile-His-Pro-Phe（ANG(3-8)）（6.55微摩尔）。将SHR血浆中ANG II的代谢行为与正常血压的Wistar血浆中的代谢行为进行比较，Wistar血浆中的初始降解速率（3.07纳摩尔/毫升/分钟）比SHR血浆中的初始降解速率（1.75纳摩尔/毫升/分钟）高约2倍。
