Murakami T, Takemori K, Yoshizumi H
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University, Nara, Japan.
Biosci Biotechnol Biochem. 1995 Aug;59(8):1459-63. doi: 10.1271/bbb.59.1459.
The incipient timing of cerebral strokes in the stroke-prone spontaneously hypertensive rats (SHRSP) was biochemically determined by investigating the relationship between the glutathione peroxidase (GSH-Px) activity in erythrocytes and the extent of stroke lesions. When the blood pressure of SHRSPs was maintained at over 240 mmHg, the GSH-Px activity fell, and the body weight also decreased. In SHRSP whose GSH-Px activity in erythrocytes had dropped below 23 units/ml of blood, the incidence of cerebral strokes was 98% (n = 88/90). The hematocrit level did not change even after the GSH-Px activity had dropped to 23 units/ml of blood. The reduced GSH-Px activity in erythrocytes observed during continued hypertension was found to be due to a decrease in GSH-Px protein, and not to any inactivation of the enzyme, as evident from immunochemical titration. At the moment when the GSH-Px activity had dropped to 23 units/ml of blood, and the control diet was changed to one based on fish or a hydralazine treatment given, the activity recovered, and an increase in body weight and prolongation of the life-span were observed. It was deduced from these findings that tracing the GSH-Px activity in erythrocytes in SHRSP would serve as an indicator for predicting and prognosing stroke lesions.
通过研究红细胞中谷胱甘肽过氧化物酶（GSH-Px）活性与中风损伤程度之间的关系，从生化角度确定了易中风自发性高血压大鼠（SHRSP）中风的起始时间。当SHRSP的血压维持在240 mmHg以上时，GSH-Px活性下降，体重也降低。红细胞中GSH-Px活性降至23单位/毫升血液以下的SHRSP，脑中风发生率为98%（n = 88/90）。即使GSH-Px活性降至23单位/毫升血液后，血细胞比容水平也没有变化。从免疫化学滴定可以明显看出，持续高血压期间观察到的红细胞中GSH-Px活性降低是由于GSH-Px蛋白减少，而非酶的任何失活。当GSH-Px活性降至23单位/毫升血液时，将对照饮食改为基于鱼类的饮食或给予肼屈嗪治疗后，活性恢复，体重增加且寿命延长。从这些发现可以推断，追踪SHRSP红细胞中的GSH-Px活性将作为预测和预后中风损伤的指标。
