易卒中型自发性高血压大鼠红细胞谷胱甘肽过氧化物酶与卒中病变发生率的关系。

Relationship between erythrocytes glutathione peroxidase and incidence of stroke lesion in the stroke-prone spontaneously hypertensive rat.

Murakami T, Takemori K, Takeda T, Yoshizumi H

Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University, Nara, Japan.

Clin Exp Pharmacol Physiol Suppl. 1995 Dec;22(1):S162-4. doi: 10.1111/j.1440-1681.1995.tb02864.x.

Abstract

To determine biochemically the incipient timing of cerebral stroke in stroke-prone spontaneously hypertensive rats (SHRSP) the relation between the glutathione peroxidase (GSH-Px) activity in erythrocytes and the extent of stroke lesion was investigated. 2. When the blood pressure of SHRSP was maintained over 250 mmHg, the GSH-Px activity was lowered and the body weight also decreased. In the SHRSP where the GSH-Px activity in erythrocytes dropped below 23 units/mL blood, the incidence of cerebral stroke was 98% (n = 88/90). 3. The haemoglobin and haematocrit level were unchanged even after the GSH-Px activity dropped to 23 units/mL blood. 4. Lowering of GSH-Px activity in erythrocytes observed during continued hypertension was found to be due to decreased GSH-Px protein, but not to an inactivation of enzymes, as evidenced from immunochemical titration. 5. Lowering of GSH-Px activity in erythrocytes was found to be closely related with the incidence of cerebral stroke in SHRSP. These findings suggest that tracing of the GSH-Px activity in erythrocytes in SHRSP may serve as an indicator for prediction and prognosis of stroke lesion.

摘要

为了从生化角度确定易患中风的自发性高血压大鼠（SHRSP）脑中风的起始时间，研究了红细胞中谷胱甘肽过氧化物酶（GSH-Px）活性与中风损伤程度之间的关系。2. 当SHRSP的血压维持在250 mmHg以上时，GSH-Px活性降低，体重也下降。在红细胞中GSH-Px活性降至23单位/毫升血液以下的SHRSP中，脑中风的发生率为98%（n = 88/90）。3. 即使GSH-Px活性降至23单位/毫升血液后，血红蛋白和血细胞比容水平仍未改变。4. 持续高血压期间观察到的红细胞中GSH-Px活性降低是由于GSH-Px蛋白减少，而非酶失活，免疫化学滴定证明了这一点。5. 发现红细胞中GSH-Px活性降低与SHRSP脑中风的发生率密切相关。这些发现表明，追踪SHRSP红细胞中的GSH-Px活性可能作为中风损伤预测和预后的指标。