Takamatsu S, Nakano K
Nagoya University Bio-Science Center, Chikusa, Japan.
Biosci Biotechnol Biochem. 1995 Aug;59(8):1493-7. doi: 10.1271/bbb.59.1493.
An injection of Escherichia coli lipopolysaccharide (LPS) increased the activity of histidine decarboxylase (HDC) in bone marrow (BM) cells of C3H/HeN mice much more than in C3H/HeJ mice, which are resistant to various effects of LPS. In WBB6/F1 (W/Wv) mice, which are genetically deficient in mast cells, HDC activity increased more than in C3H/HeN mice. Cultured BM cells of W/Wv mice spontaneously synthesized histamine in a HDC-dependent way. LPS caused a slight increase in HDC-associated histamine synthesis by these cells. Treatment of the BM cells with murine recombinant granulocyte-macrophage colony stimulating factor (mrGM-CSF) increased the histamine synthesis. In addition, treatment with mrGM-CSF made the cells respond to LPS by a dose-dependent increase in HDC activity and histamine synthesis. Most dish-adherent BM cells that had been treated with both mrGM-CSF and LPS for 48 h were stained for nonspecific esterase and not for chloroacetate esterase, and had twice as much HDC activity as the nonadherent cells had. Immunocytochemical analysis of the BM cells of W/Wv mice treated with both mrGM-CSF and LPS showed that HDC was in the cytoplasm of cells having Mac-1, a macrophage-differentiation antigen. These results suggest that cells of the macrophage lineage in the BM of mice synthesize histamine.
注射大肠杆菌脂多糖（LPS）后，C3H/HeN小鼠骨髓（BM）细胞中组氨酸脱羧酶（HDC）的活性增加幅度远大于对LPS各种效应具有抗性的C3H/HeJ小鼠。在基因上缺乏肥大细胞的WBB6/F1（W/Wv）小鼠中，HDC活性的增加幅度大于C3H/HeN小鼠。W/Wv小鼠的培养骨髓细胞以HDC依赖的方式自发合成组胺。LPS使这些细胞中与HDC相关的组胺合成略有增加。用小鼠重组粒细胞-巨噬细胞集落刺激因子（mrGM-CSF）处理骨髓细胞可增加组胺合成。此外，用mrGM-CSF处理使细胞通过HDC活性和组胺合成的剂量依赖性增加对LPS产生反应。用mrGM-CSF和LPS处理48小时的大多数贴壁骨髓细胞经非特异性酯酶染色而非氯乙酸酯酶染色，其HDC活性是非贴壁细胞的两倍。对用mrGM-CSF和LPS处理的W/Wv小鼠骨髓细胞进行免疫细胞化学分析表明，HDC存在于具有巨噬细胞分化抗原Mac-1的细胞的细胞质中。这些结果表明，小鼠骨髓中巨噬细胞系的细胞合成组胺。
