Takamatsu S, Nakano K
Nagoya University BioScience Center, Japan.
Methods Find Exp Clin Pharmacol. 1995 Nov;17 Suppl C:21-4.
Promyelocytic HL-60 cells differentiated into mature cells when they were cultured in the presence of dimaprit (10(-4) M), a histamine H2 agonist. An injection of Escherichia coli lipopolysaccharide increased the activity of histidine decarboxylase in bone marrow cells in C3H/HeN mice to a much greater extent than in C3H/HeJ mice, which are resistant to various effects of lipopolysaccharide. Histamine production increased concomitantly. In WBB6/F1 (W/W(v)) mice, which are genetically deficient in mast cells, histidine decarboxylase activity increased more than in C3H/HeN mice. Pure (>99% nonspecific esterase, CD14 and Mac-1 positive) macrophage populations were obtained from long-term culture of the bone marrow cells (bone marrow-derived macrophages, BMDM). Culture of the cells in the presence of lipopolysaccharide caused a slight, but dose-dependent increase in histidine decarboxylase-associated histamine synthesis. Granulocyte/macrophage colony-stimulating factor (rmGM-CSF) or interleukin 3 (rmIL-3) potently increased lipopolysaccharide-induced histamine formation.
早幼粒细胞性HL-60细胞在组胺H2激动剂二甲双胍（10^(-4) M）存在的情况下培养时可分化为成熟细胞。注射大肠杆菌脂多糖后，C3H/HeN小鼠骨髓细胞中组氨酸脱羧酶的活性比C3H/HeJ小鼠（对脂多糖的各种作用具有抗性）增加的程度要大得多。组胺的产生也随之增加。在基因上缺乏肥大细胞的WBB6/F1（W/W(v)）小鼠中，组氨酸脱羧酶活性的增加比C3H/HeN小鼠更多。从骨髓细胞的长期培养中获得了纯的（>99%非特异性酯酶、CD14和Mac-1阳性）巨噬细胞群体（骨髓来源的巨噬细胞，BMDM）。在脂多糖存在的情况下培养细胞会导致组氨酸脱羧酶相关的组胺合成有轻微但呈剂量依赖性的增加。粒细胞/巨噬细胞集落刺激因子（rmGM-CSF）或白细胞介素3（rmIL-3）能显著增加脂多糖诱导的组胺形成。
