Raju G M, Kochupillai V, Kumar L
Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, Ansari Nagar, New Delhi.
Natl Med J India. 1995 Sep-Oct;8(5):216-21.
For reinfusing autologous bone marrow cells after high-dose chemotherapy and/or radiotherapy it is necessary that an effective technique for their storage is available. The traditional method uses 10% dimethyl sulphoxide as cryoprotectant, a rate-controlled computerized freezer programmed to cool the cells at a constant rate of 1 degrees C per minute and liquid nitrogen as the storage system. The method is time-consuming, expensive and requires technical expertise. Moreover, it is often associated with varying levels of clinical toxicity following infusion of the preserved cells. Processing the harvest to reduce the initial volume and the mature cells has been shown to be beneficial in reducing the volume of the cryoprotectant and the incidence of toxicity. An alternative, cost-effective method using a cryoprotectant mixture of 5% dimethyl sulphoxide, 6% hydroxyethyl starch and 4% albumin has been found to be effective even when the cells are stored at -80 degrees C without rate-controlled freezing. However, its efficacy needs to be evaluated for extended periods. The current use of purging and cell sorting methods seems to be promising.
对于在大剂量化疗和/或放疗后回输自体骨髓细胞而言，具备一种有效的细胞储存技术很有必要。传统方法使用10%的二甲基亚砜作为冷冻保护剂，一台程序控制的速率冷冻机，设定以每分钟1摄氏度的恒定速率冷却细胞，并使用液氮作为储存系统。该方法耗时、昂贵且需要专业技术知识。此外，在输注保存的细胞后，它常常伴有不同程度的临床毒性。已证明对采集物进行处理以减少初始体积和成熟细胞数量，有利于减少冷冻保护剂的用量和毒性发生率。已发现一种替代的、经济有效的方法，即使用由5%二甲基亚砜、6%羟乙基淀粉和4%白蛋白组成的冷冻保护剂混合物，即使在细胞于-80摄氏度储存且无速率控制冷冻的情况下也有效。然而，其长期疗效仍需评估。目前使用的净化和细胞分选方法似乎很有前景。
