甾体皂苷对12-O-十四烷酰佛波醇-13-乙酸酯（TPA）增强的32P掺入HeLa细胞磷脂及人恶性肿瘤细胞增殖的抑制作用。

Inhibitory effects of steroidal saponins on 12-O-tetradecanoylphorbol-13-acetate (TPA)-enhanced 32P-incorporation into phospholipids of HeLa cells and proliferation of human malignant tumor cells.

作者信息

Mimaki Y, Sashida Y, Kuroda M, Nishino A, Satomi Y, Nishino H

机构信息

School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.

出版信息

Biol Pharm Bull. 1995 Mar;18(3):467-9. doi: 10.1248/bpb.18.467.

DOI:10.1248/bpb.18.467

PMID:7550106

Abstract

Certain Lilium plants contain (25S)-spirost-5-ene-3 beta,27-diol glycosides embracing 3-hydroxy-3-methylglutaric acid at the C-27 hydroxy position. One of their derivatives, methyl ester of (25R)-27-O-[(S)-3-hydroxy-3-methylglutaryl]-spirost-5-ene-3 beta,27-diol 3-O-(O-alpha-L-rhamnopyranosyl-(1-->2)-O-[beta-D-glucopyranosyl-(1-->4)] - beta-D-glucopyranoside) was found to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated 32P-incorporation into the phospholipids of human cervical cancer (HeLa) cells and also to inhibit the proliferation of various kinds of human malignant tumor cells, pancreatic cancer (PANC-1), osteosarcoma (OST), human gastric cancer (HGC-27), pheochromocytoma (PC-12) and HeLa cells, in vitro.

摘要

某些百合属植物含有（25S）-螺旋甾-5-烯-3β，27-二醇糖苷，其在C-27羟基位置含有3-羟基-3-甲基戊二酸。它们的一种衍生物，即（25R）-27-O-[(S)-3-羟基-3-甲基戊二酰基]-螺旋甾-5-烯-3β，27-二醇3-O-(O-α-L-鼠李糖基-(1→2)-O-[β-D-葡萄糖基-(1→4)]-β-D-葡萄糖苷)的甲酯，被发现可抑制12-O-十四烷酰佛波醇-13-乙酸酯（TPA）刺激的32P掺入人宫颈癌（HeLa）细胞的磷脂中，并且在体外还可抑制各种人类恶性肿瘤细胞、胰腺癌（PANC-1）、骨肉瘤（OST）、人胃癌（HGC-27）、嗜铬细胞瘤（PC-12）和HeLa细胞的增殖。