Kröber S, Ruck P, Xiao J C, Kaiserling E
Institute of Pathology, University of Tübingen, Germany.
Pathol Int. 1995 Jul;45(7):501-5. doi: 10.1111/j.1440-1827.1995.tb03492.x.
The DNA ploidy pattern of nine hepatoblastomas and three normal liver specimens was investigated by flow cytometry. Areas of unlike differentiation in the same tumor were investigated separately. Normal liver tissue and the fetal subtype were always diploid. Aneuploidy was found in the embryonal subtype. The one case of small cell tumor was also diploid. When the fetal and embryonal areas of the same tumor were analyzed together, there was always an aneuploid peak in the histogram. There are obvious differences in DNA ploidy among the various hepatoblastoma subtypes. Differences in methods used, including the failure in some studies to evaluate areas of unlike differentiation in the same tumor separately, probably account to some extent for the conflicting results of previously reported studies. When flow cytometric analysis of the DNA content of a hepatoblastoma is performed, it is important to ensure that the various types of differentiation in the tumor are represented adequately in the material investigated, especially when conclusions about the prognosis are to be based on these findings.
采用流式细胞术对9例肝母细胞瘤和3例正常肝脏标本的DNA倍体模式进行了研究。同一肿瘤中不同分化区域分别进行研究。正常肝组织和胎儿型总是二倍体。胚胎型中发现了非整倍体。1例小细胞瘤也是二倍体。当对同一肿瘤的胎儿型和胚胎型区域一起分析时，直方图中总是有一个非整倍体峰。不同肝母细胞瘤亚型之间的DNA倍体存在明显差异。所采用方法的差异，包括一些研究未能分别评估同一肿瘤中不同分化区域，可能在一定程度上解释了先前报道研究结果的相互矛盾。当对肝母细胞瘤的DNA含量进行流式细胞术分析时，重要的是要确保所研究材料中充分代表肿瘤的各种分化类型，尤其是当要根据这些发现得出关于预后的结论时。
