Rugge M, Sonego F, Pollice L, Perilongo G, Guido M, Basso G, Ninfo V, Pennelli N, Gambini C, Guglielmi M, Fabiano A, Leandro G, Keeling J W
Istituto di Anatomia Patologica, Cattedra di Istochimica & Immunoistochimica Patologica, Università di Padova, Veneto, Italy.
Liver. 1998 Apr;18(2):128-33. doi: 10.1111/j.1600-0676.1998.tb00138.x.
Hepatoblastoma (HB) is the most frequent malignant liver tumor in infancy, and both its biological features and its prognostic behavior are still under investigation. DNA content and proliferative activity of the tumor have been considered as biological parameters related to the tumor's aggressiveness. The present study attempts to investigate the possible association between histologic subtype, DNA content, and proliferative indices in HB. DNA content and the proportion of cells in the S-phase were assessed by flow cytometry in 34 cases of HB (14 prior to chemotherapy, 20 after chemotherapy), using formalin-fixed, paraffin-embedded archival samples. The proliferative cell nuclear antigen (PCNA) labeling index was also evaluated by immunohistochemistry, and both the flow cytometry (FC) and the immunohistochemical data were correlated with tumor pathology. A significant association was found between histological type, DNA content and the percentage of cells in the S-phase, with aneuploidy and the highest proportions of S-phase cells significantly associated with embryonal tumors. The PCNA labeling index was found to be significantly higher in embryonal than in fetal phenotype. The biological heterogeneity of HB is Confirmed by the different nuclear content of the fetal (diploid) and embryonal (aneuploid) epithelial components of the tumor, also ruling out the likelihood of fetal (diploid) clones deriving from the embryonal (aneuploid) neoplastic cells. Since the highly proliferative neoplastic clones (i.e., embryonal) are thought to be more sensitive to antimitotic drugs, further studies are indicated to determine the relationship between ploidy, proliferative indices and chemoresponsiveness.
肝母细胞瘤（HB）是婴儿期最常见的恶性肝肿瘤，其生物学特征和预后行为仍在研究中。肿瘤的DNA含量和增殖活性被认为是与肿瘤侵袭性相关的生物学参数。本研究旨在探讨HB的组织学亚型、DNA含量和增殖指数之间可能存在的关联。使用福尔马林固定、石蜡包埋的存档样本，通过流式细胞术对34例HB患者（14例化疗前，20例化疗后）的DNA含量和S期细胞比例进行评估。还通过免疫组织化学评估增殖细胞核抗原（PCNA）标记指数，并将流式细胞术（FC）和免疫组织化学数据与肿瘤病理学相关联。研究发现组织学类型、DNA含量和S期细胞百分比之间存在显著关联，非整倍体和S期细胞的最高比例与胚胎性肿瘤显著相关。发现胚胎型HB的PCNA标记指数显著高于胎儿型。肿瘤中胎儿（二倍体）和胚胎（非整倍体）上皮成分的不同核含量证实了HB的生物学异质性，也排除了胎儿（二倍体）克隆源自胚胎（非整倍体）肿瘤细胞的可能性。由于高度增殖的肿瘤克隆（即胚胎型）被认为对抗有丝分裂药物更敏感，因此需要进一步研究以确定倍性、增殖指数和化疗反应性之间的关系。
