Hata Y, Ishizu H, Ohmori K, Hamada H, Sasaki F, Uchino J, Inoue K, Naitoh H, Fujita M, Kobayashi T
First Department of Surgery, Hokkaido University Hospital, Sapporo, Japan.
Cancer. 1991 Dec 15;68(12):2566-70. doi: 10.1002/1097-0142(19911215)68:12<2566::aid-cncr2820681206>3.0.co;2-z.
The nuclear DNA content of 15 hepatoblastoma cases was determined in paraffin-embedded tissues by flow cytometry. The DNA index (DI) was calculated, and the ploidy pattern of nuclear DNA was estimated. The correlation between the ploidy pattern and clinicopathologic findings was studied, and the prognostic significance of the ploidy pattern was investigated. An aneuploid pattern was seen in 50% of the lesions with histologic embryonal and anaplastic types. It was not seen in the fetal type. In the tumors with combined epithelial components, the fetal-type component had a diploid pattern in all five cases. The embryonal-type component was associated with aneuploidy in two of five cases. In aneuploid tumors, vascular invasion (tumor emboli in the vessels) was observed more frequently. The prognosis of the patients with an aneuploid tumor was significantly poorer. These results indicate that nuclear DNA ploidy pattern analysis might be useful in investigating the prognosis of hepatoblastoma.
采用流式细胞术对15例石蜡包埋组织中的肝母细胞瘤病例进行核DNA含量测定。计算DNA指数（DI），并估计核DNA的倍体模式。研究倍体模式与临床病理结果之间的相关性，并探讨倍体模式的预后意义。在50%组织学类型为胚胎型和间变型的病变中可见非整倍体模式。在胎儿型病变中未见。在具有上皮成分组合的肿瘤中，所有5例胎儿型成分均为二倍体模式。5例中有2例胚胎型成分与非整倍体相关。在非整倍体肿瘤中，血管侵犯（血管内肿瘤栓子）更常见。非整倍体肿瘤患者的预后明显较差。这些结果表明，核DNA倍体模式分析可能有助于研究肝母细胞瘤的预后。
