Sanjuán R, Zueco J, Pérez J, Peñarroja C, Sentandreu R
Departament de Microbiologia, Facultat de Farmàcia, Universitat de València, Spain.
Microbiology (Reading). 1996 Aug;142 ( Pt 8):2255-62. doi: 10.1099/13500872-142-8-2255.
The topological distribution of two epitopes in the cell wall of Candida albicans, the kinetics of their incorporation into the regenerating protoplast wall, and the effect of different antibiotics upon their incorporation and localization have been studied. To do so, two monoclonal antibodies that react against an O-glycosylated mannoprotein (1B12) and against a 1,6-beta-glucan epitope (JRR1) were used. The results show that the JRR1 epitope is localized in an internal layer of the cell wall, in contrast to the 1B12 epitope, which is superficial, and that the incorporation of the JRR1 epitope into walls of regenerating protoplasts precedes that of the 1B12 epitope. The JRR1 epitope is normally found in the culture medium of control cells, but not in that of papulacandin-B-treated cells, and tunicamycin interferes with the incorporation of the 1B12 epitope into the cell walls. Finally, the results support the hypothesis that mannoproteins are not 1,6-beta-glycosylated before their secretion.
研究了白色念珠菌细胞壁中两个表位的拓扑分布、它们掺入再生原生质体壁的动力学以及不同抗生素对其掺入和定位的影响。为此，使用了两种单克隆抗体，一种针对O-糖基化甘露糖蛋白（1B12），另一种针对1,6-β-葡聚糖表位（JRR1）。结果表明，与位于表面的1B12表位不同，JRR1表位位于细胞壁的内层，并且JRR1表位掺入再生原生质体壁的过程先于1B12表位。JRR1表位通常存在于对照细胞的培养基中，但不存在于丘疹霉素-B处理细胞的培养基中，衣霉素会干扰1B12表位掺入细胞壁。最后，结果支持了甘露糖蛋白在分泌前不进行1,6-β-糖基化的假说。
