Zancanaro C, Sbarbati A, Bolner A, Accordini C, Piemonte G, Osculati F
Institute of Human Anatomy and Histology, University of Verona, Italy.
Chem Senses. 1995 Jun;20(3):329-35. doi: 10.1093/chemse/20.3.329.
The presence and content of biogenic amines in taste disk-bearing fungiform papillae of the frog, Rana esculenta, the only available model of an isolated taste organ, were verified by means of HPLC. Fungiform papillae were found to contain measurable amounts of serotonin, epinephrine and norepinephrine. The amounts of serotonin and epinephrine were significantly higher in fungiform papillae than in the general mucosa of the tongue. Moreover, the epinephrine content of fungiform papillae was found to differ across the tongue, in accordance with previous physiological studies showing an inhomogeneous response of different tongue regions to taste stimuli. Ultrastructural and histochemical investigations confirmed the presence of catecholamine and serotonin. The latter was found to be contained mainly in the basal cells of the frog taste disk. These results extend previous qualitative data on the presence of biogenic amines in taste chemoreceptors.
通过高效液相色谱法（HPLC）验证了食用蛙（Rana esculenta）味蕾状菌状乳头（这是唯一可用的孤立味觉器官模型）中生物胺的存在及含量。发现菌状乳头含有可测量的血清素、肾上腺素和去甲肾上腺素。菌状乳头中的血清素和肾上腺素含量显著高于舌部的一般黏膜。此外，根据先前的生理学研究表明不同舌区对味觉刺激的反应不均匀，发现菌状乳头的肾上腺素含量在舌部各处存在差异。超微结构和组织化学研究证实了儿茶酚胺和血清素的存在。发现血清素主要存在于蛙味蕾的基底细胞中。这些结果扩展了先前关于味觉化学感受器中生物胺存在的定性数据。
