Röhlich P, Pevzner R A
Cell Tissue Res. 1982;224(2):409-20. doi: 10.1007/BF00216883.
The epithelial surface of the taste disc on the fungiform papillae of the frog was studied by means of freeze-fracture and conventional electron microscopy. The plasma membrane of the apical process of taste receptor cells was found to exhibit a uniform population of 10-nm intramembrane particles on its fracture face P in a density of 1,000-1,800/micrometers2. In contrast to intramembrane particles in mucous cells or in other regions of the plasma membrane, these particles were observed to protrude approximately twice as high from the fracture face. Not more than 1-2% of the particles were found on fracture face E of the apical process of the taste cell. The apical regions of satellite and mucous secretory cells are also described. The occurrence of relatively large intramembrane particles in a membrane region presumed to function as the chemoreceptor area of taste cells suggests that the particles may be involved in chemoreception.
运用冷冻断裂和传统电子显微镜技术对青蛙菌状乳头味蕾的上皮表面进行了研究。发现味觉受体细胞顶端突起的质膜在其断裂面P上呈现出均匀分布的10纳米膜内颗粒，密度为1000 - 1800/平方微米。与黏液细胞或质膜其他区域的膜内颗粒不同，这些颗粒从断裂面突出的高度约为其他颗粒的两倍。在味觉细胞顶端突起的断裂面E上发现的颗粒不超过1 - 2%。还描述了卫星细胞和黏液分泌细胞的顶端区域。在推测为味觉细胞化学感受器区域的膜区出现相对较大的膜内颗粒，这表明这些颗粒可能参与化学感受过程。
