利用生物传感器检测味觉刺激期间味蕾中血清素的释放。
Using biosensors to detect the release of serotonin from taste buds during taste stimulation.
作者信息
Huang Y J, Maruyama Y, Lu K S, Pereira E, Plonsky I, Baur J E, Wu D, Roper S D
机构信息
Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, FL 33136, USA.
出版信息
Arch Ital Biol. 2005 May;143(2):87-96.
Abstract
CHO cells transfected with high-affinity 5HT receptors were used to detect and identify the release of serotonin from taste buds. Taste cells release 5HT when depolarized or when stimulated with bitter, sweet, or sour tastants. Sour- and depolarization-evoked release of 5HT from taste buds is triggered by Ca2+ influx from the extracellular fluid. In contrast, bitter- and sweet-evoked release of 5HT is triggered by Ca2+ derived from intracellular stores.
摘要
用转染了高亲和力5-羟色胺（5HT）受体的中国仓鼠卵巢（CHO）细胞来检测和识别味蕾中5-羟色胺的释放。当味觉细胞去极化时，或者用苦味、甜味或酸味剂刺激时，会释放5-羟色胺。味蕾中由酸味和去极化引起的5-羟色胺释放是由细胞外液中的钙离子内流触发的。相比之下，由苦味和甜味引起的5-羟色胺释放是由细胞内储存的钙离子触发的。
相似文献
1
Using biosensors to detect the release of serotonin from taste buds during taste stimulation.利用生物传感器检测味觉刺激期间味蕾中血清素的释放。
Arch Ital Biol. 2005 May;143(2):87-96.
2
Mouse taste buds use serotonin as a neurotransmitter.小鼠味蕾将血清素用作神经递质。
J Neurosci. 2005 Jan 26;25(4):843-7. doi: 10.1523/JNEUROSCI.4446-04.2005.
3
Norepinephrine is coreleased with serotonin in mouse taste buds.去甲肾上腺素在小鼠味蕾中与5-羟色胺共同释放。
J Neurosci. 2008 Dec 3;28(49):13088-93. doi: 10.1523/JNEUROSCI.4187-08.2008.
4
Signal transduction and information processing in mammalian taste buds.哺乳动物味蕾中的信号转导与信息处理
Pflugers Arch. 2007 Aug;454(5):759-76. doi: 10.1007/s00424-007-0247-x. Epub 2007 Apr 28.
5
Acid stimulation (sour taste) elicits GABA and serotonin release from mouse taste cells.酸刺激（酸味）会从小鼠味觉细胞中释放 GABA 和血清素。
PLoS One. 2011;6(10):e25471. doi: 10.1371/journal.pone.0025471. Epub 2011 Oct 20.
6
Calcitonin Gene-Related Peptide Reduces Taste-Evoked ATP Secretion from Mouse Taste Buds.降钙素基因相关肽可减少小鼠味蕾味觉诱发的ATP分泌。
J Neurosci. 2015 Sep 16;35(37):12714-24. doi: 10.1523/JNEUROSCI.0100-15.2015.
7
A physiologic role for serotonergic transmission in adult rat taste buds.5-羟色胺能传递在成年大鼠味蕾中的生理作用。
PLoS One. 2014 Nov 11;9(11):e112152. doi: 10.1371/journal.pone.0112152. eCollection 2014.
8
The role of pannexin 1 hemichannels in ATP release and cell-cell communication in mouse taste buds.泛素连接蛋白1半通道在小鼠味蕾中ATP释放及细胞间通讯中的作用。
Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6436-41. doi: 10.1073/pnas.0611280104. Epub 2007 Mar 26.
9
Mouse taste buds release serotonin in response to taste stimuli.小鼠味蕾会根据味觉刺激释放血清素。
Chem Senses. 2005 Jan;30 Suppl 1:i39-40. doi: 10.1093/chemse/bjh102.
10
Tastants evoke cAMP signal in taste buds that is independent of calcium signaling.味觉刺激物在味蕾中引发独立于钙信号传导的环磷酸腺苷（cAMP）信号。
Am J Physiol Cell Physiol. 2006 Aug;291(2):C237-44. doi: 10.1152/ajpcell.00303.2005. Epub 2006 Mar 1.
引用本文的文献
1
Comprehensive New Insights into Sweet Taste Transmission Mechanisms and Detection Methods.甜味传递机制与检测方法的全面新见解
Foods. 2025 Jul 7;14(13):2397. doi: 10.3390/foods14132397.
2
Optogenetic Activation of Type III Taste Cells Modulates Taste Responses.光遗传学激活 III 型味觉细胞调节味觉反应。
Chem Senses. 2020 Oct 9;45(7):533-539. doi: 10.1093/chemse/bjaa044.
3
Sensing Senses: Optical Biosensors to Study Gustation.感知感官：用于味觉研究的光学生物传感器。
Sensors (Basel). 2020 Mar 25;20(7):1811. doi: 10.3390/s20071811.
4
Age-related changes in mouse taste bud morphology, hormone expression, and taste responsivity.小鼠味蕾形态、激素表达和味觉反应的年龄相关性变化。
J Gerontol A Biol Sci Med Sci. 2012 Apr;67(4):336-44. doi: 10.1093/gerona/glr192. Epub 2011 Nov 4.
5
Differential expression of a BMP4 reporter allele in anterior fungiform versus posterior circumvallate taste buds of mice.在小鼠的前菌状和后环状味蕾中，BMP4 报告基因等位基因的差异表达。
BMC Neurosci. 2010 Oct 13;11:129. doi: 10.1186/1471-2202-11-129.
6
Ghrelin is produced in taste cells and ghrelin receptor null mice show reduced taste responsivity to salty (NaCl) and sour (citric acid) tastants.胃饥饿素由味蕾细胞产生，胃饥饿素受体基因敲除小鼠对咸（NaCl）和酸（柠檬酸）味觉刺激的味觉反应性降低。
PLoS One. 2010 Sep 14;5(9):e12729. doi: 10.1371/journal.pone.0012729.
7
Vasoactive intestinal peptide-null mice demonstrate enhanced sweet taste preference, dysglycemia, and reduced taste bud leptin receptor expression.血管活性肠肽缺失小鼠表现出增强的甜味偏好、糖代谢紊乱和味蕾瘦素受体表达减少。
Diabetes. 2010 May;59(5):1143-52. doi: 10.2337/db09-0807. Epub 2010 Feb 11.
8
Common sense about taste: from mammals to insects.味觉常识：从哺乳动物到昆虫
Cell. 2009 Oct 16;139(2):234-44. doi: 10.1016/j.cell.2009.10.001.
9
Modulation of taste sensitivity by GLP-1 signaling.胰高血糖素样肽-1信号通路对味觉敏感性的调节
J Neurochem. 2008 Jul;106(1):455-63. doi: 10.1111/j.1471-4159.2008.05397.x. Epub 2008 Jul 1.
10
Immunohistochemical localization of aromatic L-amino acid decarboxylase in mouse taste buds and developing taste papillae.芳香族L-氨基酸脱羧酶在小鼠味蕾和发育中的味乳头中的免疫组织化学定位。
Histochem Cell Biol. 2007 Apr;127(4):415-22. doi: 10.1007/s00418-006-0257-3. Epub 2007 Jan 9.
本文引用的文献
1
Communication routes within the taste bud by neurotransmitters and neuropeptides.神经递质和神经肽在味蕾内的通讯途径。
Chem Senses. 2005 Jan;30 Suppl 1:i37-8. doi: 10.1093/chemse/bjh101.
2
Gap junctions among taste bud cells in mouse fungiform papillae.小鼠菌状乳头味蕾细胞间的缝隙连接。
Chem Senses. 2005 Jan;30 Suppl 1:i35-6. doi: 10.1093/chemse/bjh100.
3
Mouse taste buds use serotonin as a neurotransmitter.小鼠味蕾将血清素用作神经递质。
J Neurosci. 2005 Jan 26;25(4):843-7. doi: 10.1523/JNEUROSCI.4446-04.2005.
4
A paracrine signaling role for serotonin in rat taste buds: expression and localization of serotonin receptor subtypes.血清素在大鼠味蕾中的旁分泌信号作用：血清素受体亚型的表达与定位
Am J Physiol Regul Integr Comp Physiol. 2004 Apr;286(4):R649-58. doi: 10.1152/ajpregu.00572.2003. Epub 2004 Jan 8.
5
Morphologic characterization of rat taste receptor cells that express components of the phospholipase C signaling pathway.表达磷脂酶C信号通路成分的大鼠味觉受体细胞的形态学特征
J Comp Neurol. 2004 Jan 12;468(3):311-21. doi: 10.1002/cne.10963.
6
Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways.甜味、苦味和鲜味的编码：不同的受体细胞共享相似的信号通路。
Cell. 2003 Feb 7;112(3):293-301. doi: 10.1016/s0092-8674(03)00071-0.
7
Expression and physiological actions of cholecystokinin in rat taste receptor cells.胆囊收缩素在大鼠味觉受体细胞中的表达及生理作用
J Neurosci. 2002 Nov 15;22(22):10018-29. doi: 10.1523/JNEUROSCI.22-22-10018.2002.
8
Transport-mediated synapses in the retina.视网膜中由转运介导的突触。
Physiol Rev. 2002 Oct;82(4):875-91. doi: 10.1152/physrev.00010.2002.
9
Adrenergic signalling between rat taste receptor cells.大鼠味觉受体细胞之间的肾上腺素能信号传导。
J Physiol. 2002 Sep 1;543(Pt 2):601-14. doi: 10.1113/jphysiol.2002.020438.
10
"Type III" cells of rat taste buds: immunohistochemical and ultrastructural studies of neuron-specific enolase, protein gene product 9.5, and serotonin.大鼠味蕾的“III型”细胞：神经元特异性烯醇化酶、蛋白基因产物9.5和5-羟色胺的免疫组织化学及超微结构研究
J Comp Neurol. 2001 Nov 5;440(1):97-108. doi: 10.1002/cne.1372.
Zotero 插件