Yamada E
Sens Processes. 1978 Dec;2(4):285-95.
Elemental analysis was made on the rod outer segments and fuscin granules in the frog eye by means of an energy-dispersive X-ray spectrometer combined with an electron microscope. The materials were prepared from the unfixed fresh retina by means of air-dried or freeze-dried cryosection, freeze-substitution, or the freeze-drying embedding method. An outer segment in the air-dried cryosection showed P, K, S, Cl, and Ca peaks. The dry section of freeze-substituted or freeze-dried embedded outer segment showed P, K, S, and Cl; K was lost in the wet section. The fuscin granules showed prominent peaks of Ca and Zn constantly, in addition to S, Cl, Mg, K, and occasionally Cu. The K peak was also lost in the wet section.
利用与电子显微镜相结合的能量色散X射线光谱仪，对蛙眼的视杆外节和褐黑色素颗粒进行了元素分析。材料通过风干或冷冻干燥冷冻切片、冷冻置换或冷冻干燥包埋法从未固定的新鲜视网膜制备而成。风干冷冻切片中的外节显示出磷、钾、硫、氯和钙的峰。冷冻置换或冷冻干燥包埋外节的干燥切片显示有磷、钾、硫和氯；钾在湿切片中消失。褐黑色素颗粒除了含有硫、氯、镁、钾，偶尔还有铜外，始终显示出突出的钙和锌峰。钾峰在湿切片中也消失了。
