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最近利用激光微探针质谱分析技术对视网膜组织中生理阳离子分布的研究。

Recent LAMMA studies of physiological cation distributions in retina tissues.

作者信息

Kaufmann R

出版信息

Scan Electron Microsc. 1980(Pt 2):641-6.

PMID:7423133
Abstract

In shock frozen and freeze dried specimens of cat and frog retina distribution patterns of physiological cations were obtained by means of laser microprobe mass analysis with special emphasis to Ca and Ba in structural elements such as photoreceptors and melanin granules. Ca was found to be distributed along single cones of frog in a characteristic bimodal fashion whereas Na and K showed a rather homogeneous concentration profil. The presence of large amounts of Ba in pigment granula was reconfirmed in retina pigment epithelium and, newly, in melanoma cells. Additional microchemical characterization concerned organic constituents of various tissue layers of the retina.

摘要

在猫和青蛙视网膜的速冻和冻干标本中,通过激光微探针质谱分析获得了生理阳离子的分布模式,特别关注了光感受器和黑色素颗粒等结构元素中的钙和钡。发现钙以一种典型的双峰方式沿着青蛙的单锥体分布,而钠和钾的浓度分布相当均匀。色素颗粒中大量钡的存在在视网膜色素上皮中再次得到证实,并且新发现黑色素瘤细胞中也有。额外的微化学表征涉及视网膜各组织层的有机成分。

相似文献

1
Recent LAMMA studies of physiological cation distributions in retina tissues.最近利用激光微探针质谱分析技术对视网膜组织中生理阳离子分布的研究。
Scan Electron Microsc. 1980(Pt 2):641-6.
2
The laser microprobe mass analyzer (LAMMA): a new instrument for biomedical microprobe analysis.激光微探针质量分析仪(LAMMA):一种用于生物医学微探针分析的新仪器。
Med Prog Technol. 1979 Jun 15;6(3):109-21.
3
Laser microprobe mass analysis: achievements and aspects.激光微探针质谱分析:成就与方面
Scan Electron Microsc. 1979(2):279-90.
4
Elemental analysis of frog outer segment and fuscin granule by means of x-ray microanalyzer.利用X射线微分析仪对青蛙视杆外节和褐脂色素颗粒进行元素分析。
Sens Processes. 1978 Dec;2(4):285-95.
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Matrix effects in secondary ion mass spectrometric analysis of biological tissue.生物组织二次离子质谱分析中的基体效应
Scan Electron Microsc. 1986(Pt 4):1277-90.
6
Localization of ions in retina by secondary ion mass spectrometry.
Exp Eye Res. 1976 May;22(5):505-18. doi: 10.1016/0014-4835(76)90188-3.
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Measuring calcium uptake and release by invertebrate photoreceptor cells by laser microprobe mass spectroscopy.通过激光微探针质谱法测量无脊椎动物光感受器细胞对钙的摄取和释放。
Scan Electron Microsc. 1980(Pt 2):647-54, 606.
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Ultrastructure and organisation of the retina and pigment epithelium in the cutlips minnow, Exoglossum maxillingua (Cyprinidae, Teleostei).切割唇米诺鱼(Exoglossum maxillingua,鲤科,硬骨鱼纲)视网膜和色素上皮的超微结构与组织
Histol Histopathol. 1996 Jan;11(1):55-69.
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Localization of calcium and barium in toad retina by secondary ion mass spectrometry.用二次离子质谱法对蟾蜍视网膜中的钙和钡进行定位
Brain Res. 1981 Sep 7;220(1):173-8. doi: 10.1016/0006-8993(81)90221-3.
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Fine structure of the retina and pigment epithelium in the creek chub, Semotilus atromaculatus (Cyprinidae, Teleostei).溪鲈(Semotilus atromaculatus,鲤科,硬骨鱼纲)视网膜和色素上皮的精细结构
Histol Histopathol. 1996 Jan;11(1):41-53.

引用本文的文献

1
Cell viability of retinal photoreceptor evaluated by polar distribution of Ca(2+) and electrical charge.通过Ca(2+)的极性分布和电荷评估视网膜光感受器的细胞活力。
J Cell Mol Med. 2001 Jul-Sep;5(3):295-302. doi: 10.1111/j.1582-4934.2001.tb00163.x.
2
Observations on the histochemistry of barium.
Histochemistry. 1983;79(1):111-6. doi: 10.1007/BF00494348.