[马桑内酯对大鼠γ-氨基丁酸分泌及谷氨酸脱羧酶及其受体结合的影响]

[Coriaria lactone on gamma-aminobutyric acid secretion and glutamic acid decarboxylase and its receptor binding in rat].

作者信息

Zhu X, Zhu C, Wu Y

机构信息

Department of Brain Research, Tongji Medical University, Wuhan.

出版信息

Zhonghua Yi Xue Za Zhi. 1995 Jun;75(6):363-5, 384.

PMID:7553151

Abstract

The effects of coriaria lactone (CL) on gamma-aminobutyric acid (GABA) secretion, glutamic acid decarboxylase (GAD) activity and glutamate (Glu) receptor binding were studied by using cultured neurons, P2 component and synaptic membranes from rat cerebral cortex. It was found that GABA secretion was depressed by CL, the depression rate was 8.3%, 10.6%, 14.5% at 12h 24h 48h respectively. GAD activity was depressed by CL in concentration of 0.15-150 mumol/L. The depression rate was 1.32%, 5.96%, 13.24%, 18.76% respectively. The Glu receptor binding capability was decreased by CL with obvious dose-effect relation in the extent of 2.8-350 mumol/L; the decrease rate was 4.4%, 12.7%, 15.2%, 19.5% respectively. The difference was significant as compared to control (P < 0.01).

摘要

采用大鼠大脑皮层培养神经元、P2组分和突触膜，研究马桑内酯（CL）对γ-氨基丁酸（GABA）分泌、谷氨酸脱羧酶（GAD）活性及谷氨酸（Glu）受体结合的影响。结果发现，CL可抑制GABA分泌，在12小时、24小时、48小时时的抑制率分别为8.3%、10.6%、14.5%。CL在0.15 - 150μmol/L浓度下可抑制GAD活性，抑制率分别为1.32%、5.96%、13.24%、18.76%。在2.8 - 350μmol/L范围内，CL可降低Glu受体结合能力，且具有明显的剂量效应关系；降低率分别为4.4%、12.7%、15.2%、19.5%。与对照组相比，差异有统计学意义（P < 0.01）。