Bar Dayan Y, Keynan N, Waisel Y, Pick A I, Tamir R
Division of Clinical Immunology and Allergy, Beilinson Medical Centre, Petah Tiqva, Israel.
Clin Exp Allergy. 1995 May;25(5):456-60. doi: 10.1111/j.1365-2222.1995.tb01077.x.
Thirty-six symptomatic patients, with positive skin reactions to Cupressus sempervirens pollen extract were skin-tested with pollen extracts of Podocarpus gracilior and Callitris verrucosa, of these 17 (47%) had positive responses to P. gracilior, nine (25%) to C. verrucosa, and six (17%) to both. None of the non-atopic healthy controls had positive reactions to either of the extracts. Radioallergosorbent test (RAST)-inhibition studies were performed with pooled sera from three patients. Fifty per cent inhibition was obtained with 11 micrograms protein of C. sempervirens, 54 micrograms of P. gracilior, and 71 micrograms of C. verrucosa; however, when pollen extract of Olea europaea, an unrelated allergen, was tested, 265 micrograms protein were needed to obtain 50% inhibition. One-dimension sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of pollen extracts from the three species revealed that they had several very similar protein bands. Using Western blot analysis, several closely related IgE binding proteins were identified in the three species. It was concluded that the pollen grains of P. gracilior and of C. verrucosa are potentially allergenic. The presence of common allergenic proteins indicate partial crossreactivity with C. sempervirens.
36名对地中海柏木花粉提取物皮肤反应呈阳性的有症状患者，用细叶罗汉松和疣皮南洋杉的花粉提取物进行皮肤试验，其中17名（47%）对细叶罗汉松呈阳性反应，9名（25%）对疣皮南洋杉呈阳性反应，6名（17%）对两者均呈阳性反应。非特应性健康对照者对任何一种提取物均无阳性反应。对3名患者的混合血清进行了放射变应原吸附试验（RAST）抑制研究。用11微克地中海柏木蛋白、54微克细叶罗汉松蛋白和71微克疣皮南洋杉蛋白可获得50%的抑制率；然而，当测试不相关变应原油橄榄的花粉提取物时，需要265微克蛋白才能获得50%的抑制率。对这三个物种的花粉提取物进行一维十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）分析，结果显示它们有几条非常相似的蛋白条带。通过蛋白质印迹分析，在这三个物种中鉴定出几种密切相关的IgE结合蛋白。得出的结论是，细叶罗汉松和疣皮南洋杉的花粉粒具有潜在的致敏性。共同变应原蛋白的存在表明与地中海柏木存在部分交叉反应性。
