Fountain D W, Berggren B, Nilsson S, Einarsson R
Botany and Zoology Department, Massey University, Palmerston North, New Zealand.
Int Arch Allergy Immunol. 1992;98(4):370-6. doi: 10.1159/000236213.
Dot immunoblotting of crude extracts of various aerial parts of birch trees, using patient serum rich in birch pollen IgE, showed IgE-binding activity in leaves, buds, twigs, seeds, bark, and old male catkins. Seed extracts analysed by SDS-PAGE, electroblotting to nitrocellulose and immune detection using isotope-conjugated anti-IgE verified the presence in seeds of an IgE-binding protein of an approximate molecular weight of 12 kD, distinct from the major allergen (molecular weight 17 kD) of Betula verrucosa pollen. The allergen of birch seeds was readily leachable from the seeds. Many of the birch plant part extracts were active in RAST inhibition using birch pollen RAST discs, but showed low potency relative to the allergenicity of birch pollen allergens.
利用富含桦树花粉IgE的患者血清，对桦树不同地上部分的粗提物进行斑点免疫印迹分析，结果显示叶片、芽、嫩枝、种子、树皮和老雄花序中存在IgE结合活性。通过SDS-PAGE分析种子提取物，将其电印迹到硝酸纤维素膜上，并用同位素偶联的抗IgE进行免疫检测，证实种子中存在一种分子量约为12 kD的IgE结合蛋白，与疣桦花粉的主要过敏原（分子量17 kD）不同。桦树种子中的过敏原很容易从种子中浸出。许多桦树植物部分提取物在使用桦树花粉RAST盘的RAST抑制试验中具有活性，但相对于桦树花粉过敏原的致敏性而言，其效力较低。
