Donati M, Graciotti C, Di Francesco A, Pavan G, Bui C, Cevenini R
Institute of Microbiology, University of Bologna, Italy.
New Microbiol. 1995 Jul;18(3):315-8.
The influence of centrifugation on the infectivity of Chlamydia pneumoniae IOL-207 was compared with seven serovars of C. trachomatis biovar trachoma, the three serovars of C. trachomatis biovar lymphogranuloma and four C. psittaci strains. Prolonged centrifugation from 1 to 4 hours resulted in a 3-fold increase in the infectivity of C. pneumoniae and variable or no increase in the infectivity of the other Chlamydia strains studied. These findings indicate that a 4 hour centrifugation of C. pneumoniae is recommended to improve the isolation procedures of this organism in cell cultures.
将肺炎衣原体IOL-207的感染性与沙眼衣原体生物变种沙眼的7个血清型、沙眼衣原体生物变种淋巴肉芽肿的3个血清型以及4株鹦鹉热衣原体菌株进行了离心对其感染性影响的比较。从1小时延长至4小时的长时间离心导致肺炎衣原体的感染性增加了3倍，而所研究的其他衣原体菌株的感染性则有不同程度的增加或无增加。这些结果表明，建议对肺炎衣原体进行4小时的离心，以改进该生物体在细胞培养中的分离程序。
