Fukuda M, Harada Y, Imahori S, Fukumasa-Nakai Y, Hayashi Y
Faculty of Agriculture, Shinshu University, Nagano, Japan.
Curr Genet. 1995 May;27(6):550-4. doi: 10.1007/BF00314446.
By using mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) as genetic markers, the modes of mitochondrial inheritance in sexual crosses and protoplast cell fusions of the higher basidiomycete Lentinula edodes were examined. All newly established dikaryons from reciprocal crosses between compatible monokaryons carrying different mtDNA RFLP phenotypes retained mtDNA genotypes from one of the monokaryons, suggesting that mitochondrial inheritance is principally uniparental. In contrast, it was shown that recombinant mtDNA genomes arose in some dikaryons obtained after protoplast cell fusion. Based on these results, a possible mechanism for mitochondrial inheritance in L. edodes is discussed.
通过使用线粒体DNA（mtDNA）限制性片段长度多态性（RFLP）作为遗传标记，研究了高等担子菌香菇有性杂交和原生质体细胞融合中线粒体的遗传模式。携带不同mtDNA RFLP表型的兼容单核体之间进行正反交所产生的所有新建立的双核体，其mtDNA基因型均保留自其中一个单核体，这表明线粒体遗传主要是单亲遗传。相比之下，研究表明在原生质体细胞融合后获得的一些双核体中出现了重组mtDNA基因组。基于这些结果，讨论了香菇线粒体遗传的一种可能机制。
