Matsumoto T, Fukumasa-Nakai Y
The Tottori Mycological Institute, 211 Kokoge, Tottori 689-11, Japan.
Curr Genet. 1996 Dec;30(6):549-52. doi: 10.1007/s002940050168.
The inheritance of mitochondrial DNA (mtDNA) in sexual crosses was investigated to expand our understanding of the large genetic divergence in mtDNAs among natural populations of the higher basidiomycete Pleurotus ostreatus. Reciprocal crosses were made between compatible monokaryons with distinguishable mtDNA restriction fragment length polymorphisms (PFLPs). Almost all of the dikaryons produced by these crosses had mtDNA genotypes from one of the parental monokaryons. However, for dikaryons isolated from the junction-zone of crossed monokaryons, recombinant mitochondrial genomes commonly appeared. These results showed that P. ostreatus mtDNA can be inherited biparentally, via mtDNA recombination, as well as uniparentally. Further, it was suggested that mtDNA recombination may be an important source of variation in mitochondrial genomes among natural populations of P. ostreatus.
为了更深入了解高等担子菌平菇自然种群中线粒体DNA（mtDNA）的巨大遗传差异，研究了其在有性杂交中的遗传情况。在具有可区分的mtDNA限制性片段长度多态性（PFLP）的兼容单核体之间进行了正反交。这些杂交产生的几乎所有双核体的mtDNA基因型都来自亲本单核体之一。然而，对于从杂交单核体交界区分离出的双核体，重组线粒体基因组普遍出现。这些结果表明，平菇的mtDNA既可以通过mtDNA重组双亲遗传，也可以单亲遗传。此外，有人认为mtDNA重组可能是平菇自然种群线粒体基因组变异的重要来源。
