Nishihara T, Ishihara Y, Koseki T, Boutsi E A, Senpuku H, Hanada N
Department of Oral Science, National Institute of Health, Tokyo, Japan.
Cytobios. 1995;81(327):229-37.
The effect of paraformaldehyde-fixed murine macrophage P388D1 cells stimulated with Actinobacillus actinomycetemcomitans Y4 lipopolysaccharide (membrane-associated interleukin-1) on osteoclastic bone resorption was investigated. Both the number of osteoclasts and the bone resorption surfaces increased considerably along the inside of the marrow space of mouse calvaria when membrane-associated interleukin-1 mediated by A. actinomycetemcomitans Y4 lipopolysaccharide were injected into the subcutaneous tissues overlying the central calvaria of 4-week-old C3H/HeJ mice. Membrane-associated interleukin-1 caused hypercalcaemia when injected into C3H/HeJ mice twice a day for 2 days. The calvaria of C3H-HeJ mice injected with membrane-associated interleukin-1 contained less mineral than those of the control mice. These results suggest that membrane-associated interleukin-1 on macrophages stimulated with A. actinomycetemcomitans Y4 lipopolysaccharide plays an important role in inflammatory osteoclastic bone resorption in periodontal diseases.
研究了用伴放线放线杆菌Y4脂多糖（膜相关白细胞介素-1）刺激的多聚甲醛固定的小鼠巨噬细胞P388D1细胞对破骨细胞骨吸收的影响。当将由伴放线放线杆菌Y4脂多糖介导的膜相关白细胞介素-1注射到4周龄C3H/HeJ小鼠颅骨中央上方的皮下组织中时，小鼠颅骨骨髓腔内的破骨细胞数量和骨吸收表面均显著增加。当每天给C3H/HeJ小鼠注射膜相关白细胞介素-1两次，持续2天时，会导致高钙血症。注射了膜相关白细胞介素-1的C3H-HeJ小鼠的颅骨所含矿物质比对照小鼠的少。这些结果表明，用伴放线放线杆菌Y4脂多糖刺激的巨噬细胞上的膜相关白细胞介素-1在牙周疾病的炎性破骨细胞骨吸收中起重要作用。
