Nakamura H, Fukusaki Y, Yoshimura A, Shiraishi C, Kishimoto M, Kaneko T, Hara Y
Department of Periodontology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Oral Microbiol Immunol. 2008 Jun;23(3):190-5. doi: 10.1111/j.1399-302X.2007.00410.x.
Few in vivo studies have demonstrated whether Toll-like receptor 4 (TLR4) is indispensable for lipopolysaccharide (LPS)-induced bone resorption and little is known about the receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoprotegerin (OPG) expression induced by LPS under conditions of lack of TLR4.
We compared bone resorption histomorphometrically in C3H/HeN and C3H/HeJ mice that were repeatedly injected with Actinobacillus actionmycetemcomitans LPS into their gingiva every 48 h. RANKL-, interleukin-1beta- and OPG-positive cells in the connective tissue were also compared immunohistochemically.
Bone resorption in C3H/HeJ mice in the fourth, seventh, and tenth injection groups was significantly less than that C3H/HeN mice (P < 0.05). The number of RANKL-positive cells in C3H/HeJ mice in the 10th injection group was significantly smaller than that in C3H/HeN mice (P < 0.05). The numbers of interleukin-1beta-positive cells in C3H/HeJ mice in the seventh and tenth injection groups were significantly decreased compared with those in C3H/HeN mice (P < 0.05). The numbers of OPG-positive cells in C3H/HeN and C3H/HeJ mice gradually increased, but there was no significant difference between the two strains of mice.
TLR4 is indispensable for LPS-induced bone resorption in vivo.
很少有体内研究证实Toll样受体4（TLR4）对于脂多糖（LPS）诱导的骨吸收是否不可或缺，并且在缺乏TLR4的情况下，对于LPS诱导的核因子κB受体激活剂配体（RANKL）和骨保护素（OPG）表达知之甚少。
我们通过组织形态计量学比较了每48小时向牙龈反复注射伴放线放线杆菌LPS的C3H/HeN和C3H/HeJ小鼠的骨吸收情况。还通过免疫组织化学比较了结缔组织中RANKL、白细胞介素-1β和OPG阳性细胞。
在第四次、第七次和第十次注射组中，C3H/HeJ小鼠的骨吸收明显少于C3H/HeN小鼠（P < 0.05）。第十次注射组中C3H/HeJ小鼠的RANKL阳性细胞数量明显少于C3H/HeN小鼠（P < 0.05）。与C3H/HeN小鼠相比，第七次和第十次注射组中C3H/HeJ小鼠的白细胞介素-1β阳性细胞数量明显减少（P < 0.05）。C3H/HeN和C3H/HeJ小鼠中OPG阳性细胞数量逐渐增加，但两品系小鼠之间无显著差异。
TLR4对于体内LPS诱导的骨吸收是不可或缺的。
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