LY295337对念珠菌属临床分离株的体外抗真菌活性

Antifungal activity of LY295337 in vitro against clinical isolates of Candida spp.

作者信息

Aller A I, Martin Mazuelos E, Morilla M D, Montero O

机构信息

Valme University Hospital, Sevilla, Spain.

出版信息

Chemotherapy. 1995 Jul-Aug;41(4):276-80. doi: 10.1159/000239356.

DOI:10.1159/000239356

PMID:7555208

Abstract

LY295337 is a new antifungal agent derived from the echinocandins; it shows in vitro and in vivo activity against a great variety of pathogenic fungi. In this study, we evaluated the in vitro activity of LY295337 against 201 strains of Candida spp. using two culture media: Sabouraud dextrose broth (SDB) and RPMI 1640; fluconazole was used as reference antifungal agent. The minimum inhibitory concentration (MIC) was determined by the broth microdilution method for LY295337 in SDB and RPMI 1640, and for fluconazole by an agar dilution method. The fungicidal activity of LY295337 was also determined. The MIC-50 as well as MIC-90 values obtained for LY295337 in SDB (range 0.025-0.6 mg/l) were lower than those obtained for RPMI 1640 (0.035-1.25 mg/l). All the strains resistant to fluconazole were sensitive to LY295337 (MIC < 0.6 mg/l). These results show that LY295337 is an excellent antifungal agent with a fungicidal activity in vitro against various species of Candida.

摘要

LY295337是一种源自棘白菌素的新型抗真菌药物；它在体外和体内对多种致病真菌均表现出活性。在本研究中，我们使用两种培养基：沙氏葡萄糖肉汤（SDB）和RPMI 1640，评估了LY295337对201株念珠菌属菌株的体外活性；氟康唑用作参考抗真菌药物。通过肉汤微量稀释法测定LY295337在SDB和RPMI 1640中的最低抑菌浓度（MIC），通过琼脂稀释法测定氟康唑的MIC。还测定了LY295337的杀菌活性。LY295337在SDB中获得的MIC-50以及MIC-90值（范围为0.025 - 0.6 mg/l）低于在RPMI 1640中获得的值（0.035 - 1.25 mg/l）。所有对氟康唑耐药的菌株对LY295337敏感（MIC < 0.6 mg/l）。这些结果表明，LY295337是一种出色的抗真菌药物，在体外对各种念珠菌具有杀菌活性。