• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

转运RNA（tRNA）被导入布氏锥虫的线粒体，这一过程与其基因组背景和遗传起源无关。

tRNAs are imported into mitochondria of Trypanosoma brucei independently of their genomic context and genetic origin.

作者信息

Hauser R, Schneider A

机构信息

Department of Biochemistry, University of Basel, Switzerland.

出版信息

EMBO J. 1995 Sep 1;14(17):4212-20. doi: 10.1002/j.1460-2075.1995.tb00095.x.

DOI:10.1002/j.1460-2075.1995.tb00095.x
PMID:7556062
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC394504/
Abstract

The mitochondrial genome of Trypanosoma brucei does not encode any identifiable tRNAs. Instead, mitochondrial tRNAs are synthesized in the nucleus and subsequently imported into mitochondria. In order to analyse the signals which target the tRNAs into the mitochondria, an in vivo import system has been developed: tRNA variants were expressed episomally and their import into mitochondria assessed by purification and nuclease treatment of the mitochondrial fraction. Three tRNA genes were tested in this system: (i) a mutated version of the trypanosomal tRNA(Tyr); (ii) a cytosolic tRNA(His) of yeast; and (iii) a human cytosolic tRNA(Lys). The tRNAs were expressed in their own genomic context, or containing various lengths of the 5'-flanking sequence of the trypanosomal tRNA(Tyr) gene. In all cases efficient import of each of the tRNAs was observed. We independently confirmed the mitochondrial import of the yeast tRNA(His), since in organello [alpha-32P]ATP-labelling of the 3'-end of the tRNA was inhibited by carboxyatractyloside, a highly specific inhibitor of the mitochondrial adenine nucleotide translocator. Import of heterologous tRNAs in their own genomic contexts supports the conclusion that no specific targeting signals are necessary to import tRNAs into mitochondria of T. brucei, but rather that the tRNA structure itself is sufficient to specify import.

摘要

布氏锥虫的线粒体基因组不编码任何可识别的tRNA。相反，线粒体tRNA在细胞核中合成，随后导入线粒体。为了分析将tRNA靶向导入线粒体的信号，已开发出一种体内导入系统：tRNA变体以附加型方式表达，并通过纯化线粒体部分并进行核酸酶处理来评估其导入线粒体的情况。在该系统中测试了三个tRNA基因：(i) 布氏锥虫tRNA(Tyr)的突变版本；(ii) 酵母的胞质tRNA(His)；(iii) 人的胞质tRNA(Lys)。这些tRNA在其自身的基因组环境中表达，或包含布氏锥虫tRNA(Tyr)基因不同长度的5'侧翼序列。在所有情况下，均观察到每种tRNA的有效导入。我们独立证实了酵母tRNA(His)的线粒体导入，因为线粒体腺嘌呤核苷酸转运体的高度特异性抑制剂羧基苍术苷抑制了tRNA 3'端在细胞器内的[α-32P]ATP标记。在其自身基因组环境中导入异源tRNA支持这样的结论，即布氏锥虫线粒体导入tRNA不需要特定的靶向信号，而是tRNA结构本身就足以确定导入。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/16b49aeee01f/emboj00041-0104-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/37e805ea9129/emboj00041-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/8e3fc6313ab8/emboj00041-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/02e492d69644/emboj00041-0102-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/8cd4f2e3dc8e/emboj00041-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/cea2e779a977/emboj00041-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/9f2f1f64ba07/emboj00041-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/16b49aeee01f/emboj00041-0104-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/37e805ea9129/emboj00041-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/8e3fc6313ab8/emboj00041-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/02e492d69644/emboj00041-0102-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/8cd4f2e3dc8e/emboj00041-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/cea2e779a977/emboj00041-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/9f2f1f64ba07/emboj00041-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdeb/394504/16b49aeee01f/emboj00041-0104-c.jpg

相似文献

1
tRNAs are imported into mitochondria of Trypanosoma brucei independently of their genomic context and genetic origin.转运RNA（tRNA）被导入布氏锥虫的线粒体，这一过程与其基因组背景和遗传起源无关。
EMBO J. 1995 Sep 1;14(17):4212-20. doi: 10.1002/j.1460-2075.1995.tb00095.x.
2
Cytosolic yeast tRNA(His) is covalently modified when imported into mitochondria of Trypanosoma brucei.胞质酵母tRNA（His）在导入布氏锥虫线粒体时会发生共价修饰。
Nucleic Acids Res. 1996 Apr 1;24(7):1225-8. doi: 10.1093/nar/24.7.1225.
3
tRNAs and proteins are imported into mitochondria of Trypanosoma brucei by two distinct mechanisms.转运RNA（tRNAs）和蛋白质通过两种不同机制被导入布氏锥虫的线粒体。
Mol Biol Cell. 1999 Aug;10(8):2547-57. doi: 10.1091/mbc.10.8.2547.
4
A nuclear encoded tRNA of Trypanosoma brucei is imported into mitochondria.布氏锥虫的一种核编码tRNA被导入线粒体。
Mol Cell Biol. 1994 Apr;14(4):2317-22. doi: 10.1128/mcb.14.4.2317-2322.1994.
5
tRNAs in Trypanosoma brucei: genomic organization, expression, and mitochondrial import.布氏锥虫中的转运RNA：基因组组织、表达及线粒体导入
Mol Cell Biol. 2002 Jun;22(11):3707-17. doi: 10.1128/MCB.22.11.3707-3716.2002.
6
Identification of nuclear encoded precursor tRNAs within the mitochondrion of Trypanosoma brucei.布氏锥虫线粒体内核编码前体tRNA的鉴定。
J Biol Chem. 1992 Nov 25;267(33):23963-71.
7
Thiolated tRNAs of Trypanosoma brucei are imported into mitochondria and dethiolated after import.布氏锥虫的硫代 tRNA 被导入线粒体，并在导入后进行脱硫。
J Biol Chem. 2009 Dec 25;284(52):36491-36499. doi: 10.1074/jbc.M109.064527. Epub 2009 Oct 29.
8
Nuclear-encoded mitochondrial tRNAs of Trypanosoma brucei have a modified cytidine in the anticodon loop.布氏锥虫的核编码线粒体tRNA在反密码子环中有一个修饰的胞嘧啶。
Nucleic Acids Res. 1994 Sep 11;22(18):3699-705. doi: 10.1093/nar/22.18.3699.
9
In vitro import of a nuclearly encoded tRNA into the mitochondrion of Trypanosoma brucei.核编码的tRNA体外导入布氏锥虫线粒体的研究
Mol Cell Biol. 1999 Sep;19(9):6253-9. doi: 10.1128/MCB.19.9.6253.
10
Selective import of nuclear-encoded tRNAs into mitochondria of the protozoan Leishmania tarentolae.核编码tRNA选择性导入原生动物塔氏利什曼原虫线粒体。
Mol Biochem Parasitol. 1993 Apr;58(2):233-45. doi: 10.1016/0166-6851(93)90045-y.

引用本文的文献

1
Localization of RNAs to the mitochondria-mechanisms and functions.RNAs 定位于线粒体：机制与功能。
RNA. 2024 May 16;30(6):597-608. doi: 10.1261/rna.079999.124.
2
Inducible orthogonal aminoacylation demonstrates that charging is required for mitochondrial tRNA import in Trypanosoma brucei.诱导正交氨酰化表明在布氏锥虫中线粒体 tRNA 输入需要充电。
Sci Rep. 2019 Jul 25;9(1):10836. doi: 10.1038/s41598-019-47268-4.
3
tRNAs and proteins use the same import channel for translocation across the mitochondrial outer membrane of trypanosomes.

本文引用的文献

1
Import of RNA into mitochondria.RNA导入线粒体。
Trends Cell Biol. 1994 Aug;4(8):282-6. doi: 10.1016/0962-8924(94)90218-6.
2
Identification of a tRNA-like molecule that copurifies with the 7SL RNA of Trypanosoma brucei.与布氏锥虫7SL RNA共纯化的类似tRNA分子的鉴定。
Mol Biochem Parasitol. 1993 Feb;57(2):223-9. doi: 10.1016/0166-6851(93)90198-7.
3
Splicing and 3'-processing of the tyrosine tRNA of Trypanosoma brucei.布氏锥虫酪氨酸tRNA的剪接与3'加工
tRNAs 和蛋白质使用相同的进口通道在原生动物线粒体的外膜间移位。
Proc Natl Acad Sci U S A. 2017 Sep 12;114(37):E7679-E7687. doi: 10.1073/pnas.1711430114. Epub 2017 Aug 28.
4
Nuclear RNase P of Trypanosoma brucei: a single protein in place of the multicomponent RNA-protein complex.布氏锥虫核 RNase P：一种替代多组分 RNA-蛋白复合物的单一蛋白。
Cell Rep. 2012 Jul 26;2(1):19-25. doi: 10.1016/j.celrep.2012.05.021. Epub 2012 Jun 28.
5
Of P and Z: mitochondrial tRNA processing enzymes.关于P和Z：线粒体tRNA加工酶。
Biochim Biophys Acta. 2012 Sep-Oct;1819(9-10):1017-26. doi: 10.1016/j.bbagrm.2011.11.003. Epub 2011 Nov 23.
6
A mechanism for functional segregation of mitochondrial and cytosolic genetic codes.线粒体和胞质遗传密码功能分离的一种机制。
Proc Natl Acad Sci U S A. 2009 Nov 17;106(46):19420-5. doi: 10.1073/pnas.0909937106. Epub 2009 Oct 30.
7
Thiolated tRNAs of Trypanosoma brucei are imported into mitochondria and dethiolated after import.布氏锥虫的硫代 tRNA 被导入线粒体，并在导入后进行脱硫。
J Biol Chem. 2009 Dec 25;284(52):36491-36499. doi: 10.1074/jbc.M109.064527. Epub 2009 Oct 29.
8
Delivery of drugs and macromolecules to mitochondria.药物和大分子向线粒体的递送。
Adv Drug Deliv Rev. 2007 Aug 10;59(8):729-38. doi: 10.1016/j.addr.2007.06.004. Epub 2007 Jun 28.
9
The T-stem determines the cytosolic or mitochondrial localization of trypanosomal tRNAsMet.T 茎决定了锥虫甲硫氨酸转运 RNA（tRNAsMet）的胞质或线粒体定位。
Mol Biol Cell. 2004 Jun;15(6):2750-7. doi: 10.1091/mbc.e03-11-0821. Epub 2004 Apr 2.
10
A sequence motif within trypanosome precursor tRNAs influences abundance and mitochondrial localization.锥虫前体tRNA中的一个序列基序影响丰度和线粒体定位。
Mol Cell Biol. 2003 Dec;23(24):9061-72. doi: 10.1128/MCB.23.24.9061-9072.2003.
J Biol Chem. 1993 Oct 15;268(29):21868-74.
4
A conserved stem-loop structure in the 3' untranslated region of procyclin mRNAs regulates expression in Trypanosoma brucei.原环素mRNA 3'非翻译区中的保守茎环结构调节布氏锥虫中的表达。
Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):370-4. doi: 10.1073/pnas.91.1.370.
5
A nuclear encoded tRNA of Trypanosoma brucei is imported into mitochondria.布氏锥虫的一种核编码tRNA被导入线粒体。
Mol Cell Biol. 1994 Apr;14(4):2317-22. doi: 10.1128/mcb.14.4.2317-2322.1994.
6
Import of small RNAs into Leishmania mitochondria in vitro.体外将小RNA导入利什曼原虫线粒体
Nucleic Acids Res. 1994 Aug 25;22(16):3381-6. doi: 10.1093/nar/22.16.3381.
7
Mitochondrial minicircle DNA supports plasmid replication and maintenance in nuclei of Trypanosoma brucei.线粒体小环DNA支持布氏锥虫细胞核中的质粒复制与维持。
Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5962-6. doi: 10.1073/pnas.91.13.5962.
8
An intact protein translocating machinery is required for mitochondrial import of a yeast cytoplasmic tRNA.酵母细胞质tRNA的线粒体导入需要完整的蛋白质转运机制。
J Mol Biol. 1995 Jan 27;245(4):315-23. doi: 10.1006/jmbi.1994.0026.
9
Nuclear-encoded mitochondrial tRNAs of Trypanosoma brucei have a modified cytidine in the anticodon loop.布氏锥虫的核编码线粒体tRNA在反密码子环中有一个修饰的胞嘧啶。
Nucleic Acids Res. 1994 Sep 11;22(18):3699-705. doi: 10.1093/nar/22.18.3699.
10
In vivo expression and mitochondrial import of normal and mutated tRNA(thr) in Leishmania.利什曼原虫中正常和突变型tRNA(thr)的体内表达及线粒体导入
Mol Biochem Parasitol. 1994 Mar;64(1):121-33. doi: 10.1016/0166-6851(94)90140-6.