Acosta D, Anuforo D
Drug Chem Toxicol. 1977;1(1):19-24. doi: 10.3109/01480547709034424.
The cytotoxic effect of caffeine on heart cells was evaluated in primary cultures of rat heart muscle (M) and endothelioid (E) cells. The cultures were exposed to 5, 10, or 20 mM caffeine for 6, 12, or 24 hours. As an index of cytotoxicity produced by the treatments, in situ changes in mitochondrial membrane fragility were measured by a sensitive cytochemical technique. The 24 hr treatment with 20 mM caffeine was lethal to both E and M cell cultures; only the 12 hr treatment of M cell cultures with 20 mM caffeine produced significant labilization of mitochondrial membranes. The results of tests with other concentrations and durations of treatment were not statistically significant.
在大鼠心肌（M）细胞和类内皮（E）细胞的原代培养物中评估了咖啡因对心脏细胞的细胞毒性作用。将培养物暴露于5、10或20 mM咖啡因中6、12或24小时。作为处理产生的细胞毒性指标，通过灵敏的细胞化学技术测量线粒体膜脆性的原位变化。用20 mM咖啡因处理24小时对E细胞和M细胞培养物均具有致死性；仅用20 mM咖啡因处理M细胞培养物12小时会使线粒体膜产生明显的不稳定。其他浓度和处理持续时间的测试结果无统计学意义。
