Acosta D, Anuforo D
Toxicology. 1976 Aug-Sep;6(2):225-33. doi: 10.1016/0300-483x(76)90024-x.
The cytotoxic effect of caffeine on myocardial cells was evaluated in primary cultures of rat heart muscle (M) and endothelioid (E) cells. The cells were exposed to 5, 10, or 20 mM caffeine for 6, 12, or 24 h. As an index of cell injury produced by the treatments, in situ changes in lysosomal membrane permeability were measured by a sensitive cytochemical technique. The highest concentration of caffeine (20 mM) produced such toxic signs as reduced cell viability, increased vacuolization, and pseudopod formation. At the lower concentrations, caffeine labilized lysosomal membranes of M cells more easily than those of E cells. Pretreatment with hydrocortisone prior to exposure to caffeine protected E and M cell lysosomes from the labilizing effects of caffeine.
在大鼠心肌（M）细胞和内皮样（E）细胞的原代培养物中评估了咖啡因对心肌细胞的细胞毒性作用。将细胞暴露于5、10或20 mM咖啡因中6、12或24小时。作为处理产生的细胞损伤指标，通过灵敏的细胞化学技术测量溶酶体膜通透性的原位变化。最高浓度的咖啡因（20 mM）产生了诸如细胞活力降低、空泡化增加和伪足形成等毒性迹象。在较低浓度下，咖啡因使M细胞的溶酶体膜比E细胞的溶酶体膜更容易不稳定。在暴露于咖啡因之前用氢化可的松预处理可保护E和M细胞的溶酶体免受咖啡因的不稳定作用。
