小牛胸腺载体DNA中一个意外的转化基因：牛hst。

An unexpected transforming gene in calf-thymus carrier DNA: bovine hst.

作者信息

Yu J C, DeSeabra A J, Wang L M, Fleming T P, Chedid M, Miki T, Heidaran M A

机构信息

Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA.

出版信息

Gene. 1995 Sep 11;162(2):333-4. doi: 10.1016/0378-1119(95)00330-9.

DOI:10.1016/0378-1119(95)00330-9

PMID:7557455

Abstract

During a search for transforming genes by transfecting a human cDNA expression library together with calf thymus carrier-DNA into NIH/3T3 cells, we found a focus which was induced by a plasmid containing a sequence highly homologous to human HST (a transforming gene from Human STomach cancer). However, PCR analysis identified the source of this sequence as calf thymus DNA. The deduced amino acid (aa) sequence of bovine HST shows 91 and 81% identity to the human and mouse HST aa sequences, respectively. These data suggest that the hst of calf thymus carrier-DNA could induce transformation of NIH/3T3 cells.

摘要

在通过将人cDNA表达文库与小牛胸腺载体DNA共转染到NIH/3T3细胞中来寻找转化基因的过程中，我们发现了一个焦点，它是由一个含有与人类HST（一种来自人类胃癌的转化基因）高度同源序列的质粒诱导产生的。然而，PCR分析确定该序列的来源是小牛胸腺DNA。推导的牛HST氨基酸（aa）序列与人类和小鼠HST的aa序列分别具有91%和81%的同一性。这些数据表明，小牛胸腺载体DNA的hst可以诱导NIH/3T3细胞的转化。