Taira M, Yoshida T, Miyagawa K, Sakamoto H, Terada M, Sugimura T
Proc Natl Acad Sci U S A. 1987 May;84(9):2980-4. doi: 10.1073/pnas.84.9.2980.
The hst gene was originally identified as a transforming gene in DNAs from human stomach cancers and from a noncancerous portion of stomach mucosa by DNA-mediated transfection assay using NIH3T3 cells. cDNA clones of hst were isolated from the cDNA library constructed from poly(A)+ RNA of a secondary transformant induced by the DNA from a stomach cancer. The sequence analysis of the hst cDNA revealed the presence of two open reading frames. When this cDNA was inserted into an expression vector containing the simian virus 40 promoter, it efficiently induced the transformation of NIH3T3 cells upon transfection. It was found that one of the reading frames, which coded for 206 amino acids, was responsible for the transforming activity.
hst基因最初是通过使用NIH3T3细胞的DNA介导转染试验,在人胃癌和胃黏膜非癌部分的DNA中作为一种转化基因被鉴定出来的。hst的cDNA克隆是从由胃癌DNA诱导的二次转化体的poly(A)+ RNA构建的cDNA文库中分离出来的。hst cDNA的序列分析显示存在两个开放阅读框。当将该cDNA插入含有猿猴病毒40启动子的表达载体中时,转染后它能有效地诱导NIH3T3细胞的转化。发现其中一个编码206个氨基酸的阅读框负责转化活性。