Sango K, Johnson O N, Kozak C A, Proia R L
Section on Biochemical Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Genomics. 1995 May 20;27(2):362-5. doi: 10.1006/geno.1995.1058.
beta-1,4-N-Acetylgalactosaminyltransferase (EC 2.4.1.92; GalNAc-T) is a glycosyltransferase involved in the synthesis of gangliosides GM2 and GD2 as well as glycolipid GA2. We have isolated and sequenced the mouse Gal-NAc-T cDNA, studied GalNAc-T mRNA expression in adult tissues and in embryos, and determined the chromosomal location of the GalNAc-T gene, Ggm2. In comparison with the human cDNA, the mouse sequence was 83 and 87% identical at the nucleic acid and amino acid levels, respectively. The GalNAc-T transcript was most abundantly expressed in brain, liver, lung, spleen, and testis among the eight adult tissues examined. Relatively high levels of expression were seen early in mouse development (7-day embryos) compared to later times (11, 15, and 17 days). The Ggm2 gene was mapped to a distal position on mouse chromosome 10 that is homologous to a portion of human chromosome 12.
β-1,4-N-乙酰半乳糖胺基转移酶（EC 2.4.1.92；GalNAc-T）是一种糖基转移酶，参与神经节苷脂GM2和GD2以及糖脂GA2的合成。我们已分离并测序了小鼠Gal-NAc-T cDNA，研究了GalNAc-T mRNA在成年组织和胚胎中的表达，并确定了GalNAc-T基因Ggm2的染色体定位。与人类cDNA相比，小鼠序列在核酸和氨基酸水平上的同源性分别为83%和87%。在所检测的八个成年组织中，GalNAc-T转录本在脑、肝、肺、脾和睾丸中表达最为丰富。与发育后期（11、15和17天）相比，在小鼠发育早期（7天胚胎）可见相对较高水平的表达。Ggm2基因定位于小鼠10号染色体的远端位置，该位置与人类12号染色体的一部分同源。
