Kozak C A, Adamson M C, Buckler C E, Segovia L, Paralkar V, Wistow G
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Genomics. 1995 Jun 10;27(3):405-11. doi: 10.1006/geno.1995.1070.
The single functional mouse gene for MIF (macrophage migration inhibitory factor) has been cloned from a P1 library, and its exon/intron structure determined and shown to resemble that of the human gene. The gene was mapped to chromosome 10 using two multilocus crosses between laboratory strains and either Mus musculus musculus or Mus spretus. Nine additional loci containing related sequences, apparently all processed pseudogenes, were also mapped to chromosomes 1, 2, 3, 7, 8, 9, 12, 17, and 19. While most of these pseudogenes were found in inbred mice and M. spretus, some are species specific. This suggests that there have been active phases of pseudogene formation in Mus both before and after the separation of musculus and spretus. The human genome contains no pseudogenes; we assigned the human gene to chromosome 19, consistent with the location of mouse and human functional genes for MIF in a region of conserved linkage.
巨噬细胞移动抑制因子（MIF）的单个功能性小鼠基因已从一个P1文库中克隆出来，其外显子/内含子结构已确定，且显示与人类基因相似。利用实验室品系与小家鼠或西班牙小鼠之间的两个多位点杂交，将该基因定位到了10号染色体上。另外九个含有相关序列的位点，显然都是加工过的假基因，也被定位到了1、2、3、7、8、9、12、17和19号染色体上。虽然这些假基因大多在近交小鼠和西班牙小鼠中发现，但有些是物种特异性的。这表明在小家鼠和西班牙小鼠分化之前和之后，小家鼠中都有假基因形成的活跃阶段。人类基因组中没有假基因；我们将人类基因定位到19号染色体上，这与小鼠和人类MIF功能基因在保守连锁区域中的定位一致。
