Lyu M S, Park D J, Rhee S G, Kozak C A
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Mamm Genome. 1996 Jul;7(7):501-4. doi: 10.1007/s003359900151.
To determine chromosome positions for 10 mouse phospholipase C (PLC) genes, we typed the progeny of two sets of genetic crosses for inheritance of restriction enzyme polymorphisms of each PLC. Four mouse chromosomes, Chr 1, 11, 12, and 19, contained single PLC genes. Four PLC loci, Plcb1, Plcb2, Plcb4, and Plcg1, mapped to three sites on distal mouse Chr 2. Two PLC genes, Plcd1 and Plcg2, mapped to distinct sites on Chr 8. We mapped the human homologs of eight of these genes to six chromosomes by analysis of human x rodent somatic cell hybrids. The map locations of seven of these genes were consistent with previously defined regions of conserved synteny; Plcd1 defines a new region of homology between human Chr 3 and mouse Chr 8.
为了确定10个小鼠磷脂酶C（PLC）基因的染色体位置，我们对两组遗传杂交的后代进行了分型，以分析每个PLC的限制性酶切多态性的遗传情况。四条小鼠染色体，即1号、11号、12号和19号染色体，各含有一个PLC基因。四个PLC基因座，即Plcb1、Plcb2、Plcb4和Plcg1，定位于小鼠2号染色体远端的三个位点。两个PLC基因，即Plcd1和Plcg2，定位于8号染色体上不同的位点。通过对人-啮齿类体细胞杂种的分析，我们将其中8个基因的人类同源基因定位到了6条染色体上。其中7个基因的图谱位置与先前定义的保守同线性区域一致；Plcd1确定了人类3号染色体和小鼠8号染色体之间一个新的同源区域。
