泛素在肝癌细胞中的免疫电子显微镜定位
Immunoelectron microscopic localization of ubiquitin in hepatoma cells.
作者信息
Schwartz A L, Ciechanover A, Brandt R A, Geuze H J
机构信息
Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110.
出版信息
EMBO J. 1988 Oct;7(10):2961-6. doi: 10.1002/j.1460-2075.1988.tb03158.x.
Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.
泛素是一种由76个氨基酸组成的蛋白质，它会共价连接到异常和寿命较短的蛋白质上，从而在真核细胞中标记这些蛋白质以便进行依赖ATP的蛋白水解。使用亲和纯化的抗泛素抗体和胶体金免疫电子显微镜技术，在肝癌细胞中定位了游离（未结合）泛素。抗泛素抗体仅识别未结合的泛素。泛素存在于细胞质、细胞核、微绒毛、自噬泡和溶酶体中。
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