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泛素在肝癌细胞中的免疫电子显微镜定位

Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

作者信息

Schwartz A L, Ciechanover A, Brandt R A, Geuze H J

机构信息

Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110.

出版信息

EMBO J. 1988 Oct;7(10):2961-6. doi: 10.1002/j.1460-2075.1988.tb03158.x.

DOI:10.1002/j.1460-2075.1988.tb03158.x
PMID:2846272
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC454677/
Abstract

Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.

摘要

泛素是一种由76个氨基酸组成的蛋白质，它会共价连接到异常和寿命较短的蛋白质上，从而在真核细胞中标记这些蛋白质以便进行依赖ATP的蛋白水解。使用亲和纯化的抗泛素抗体和胶体金免疫电子显微镜技术，在肝癌细胞中定位了游离（未结合）泛素。抗泛素抗体仅识别未结合的泛素。泛素存在于细胞质、细胞核、微绒毛、自噬泡和溶酶体中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/ed22a6c599e0/emboj00147-0029-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/fb894e5002f1/emboj00147-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/807382da4390/emboj00147-0026-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/7fd028def17b/emboj00147-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/4f238c8b08b9/emboj00147-0027-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/359d850e3b4a/emboj00147-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/5eb9b3057831/emboj00147-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/ed22a6c599e0/emboj00147-0029-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/fb894e5002f1/emboj00147-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/807382da4390/emboj00147-0026-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/7fd028def17b/emboj00147-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/4f238c8b08b9/emboj00147-0027-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/359d850e3b4a/emboj00147-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/5eb9b3057831/emboj00147-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97b2/454677/ed22a6c599e0/emboj00147-0029-b.jpg

相似文献

1
Immunoelectron microscopic localization of ubiquitin in hepatoma cells.泛素在肝癌细胞中的免疫电子显微镜定位
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Small ubiquitin-related modifier 2/3 interacts with p65 and stabilizes it in the cytoplasm in HBV-associated hepatocellular carcinoma.小泛素相关修饰因子2/3与p65相互作用并使其在乙肝相关肝细胞癌的细胞质中保持稳定。
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The ubiquitin-activating enzyme, E1, is required for stress-induced lysosomal degradation of cellular proteins.泛素激活酶E1是应激诱导的细胞蛋白溶酶体降解所必需的。
J Biol Chem. 1991 Feb 25;266(6):3602-10.
6
Immunofluorescent localization of the ubiquitin-activating enzyme, E1, to the nucleus and cytoskeleton.泛素激活酶E1在细胞核和细胞骨架中的免疫荧光定位。
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Activation of the ATP-ubiquitin-proteasome pathway in skeletal muscle of cachectic rats bearing a hepatoma.荷肝癌恶病质大鼠骨骼肌中ATP-泛素-蛋白酶体途径的激活。
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Increased ubiquitin immunoreactivity in hepatocellular carcinomas and precancerous lesions of the liver.肝细胞癌及肝脏癌前病变中泛素免疫反应性增强。
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[Localization of c-myc gene protein in hepatoma cells].[肝癌细胞中c-myc基因蛋白的定位]
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Ubiquitinated aldolase B accumulates during starvation-induced lysosomal proteolysis.泛素化的醛缩酶B在饥饿诱导的溶酶体蛋白水解过程中积累。
J Cell Physiol. 1999 Jan;178(1):17-27. doi: 10.1002/(SICI)1097-4652(199901)178:1<17::AID-JCP3>3.0.CO;2-R.

引用本文的文献

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Taking Out TB-Lysosomal Trafficking and Mycobactericidal Ubiquitin-Derived Peptides.去除结核杆菌——溶酶体运输与具有杀分枝杆菌活性的泛素衍生肽
Front Microbiol. 2011 Jan 31;2:7. doi: 10.3389/fmicb.2011.00007. eCollection 2011.
2
The spatial and temporal organization of ubiquitin networks.泛素网络的时空组织。
Nat Rev Mol Cell Biol. 2011 May;12(5):295-307. doi: 10.1038/nrm3099. Epub 2011 Mar 30.
3
Ubiquitin accumulation in autophagy-deficient mice is dependent on the Nrf2-mediated stress response pathway: a potential role for protein aggregation in autophagic substrate selection.

本文引用的文献

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Biosynthesis of the human asialoglycoprotein receptor.人去唾液酸糖蛋白受体的生物合成
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Immunochemical analysis of the turnover of ubiquitin-protein conjugates in intact cells. Relationship to the breakdown of abnormal proteins.完整细胞中泛素 - 蛋白质缀合物周转的免疫化学分析。与异常蛋白质分解的关系。
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Neurolysosomal pathology in human prosaposin deficiency suggests essential neurotrophic function of prosaposin.人类鞘脂激活蛋白原缺乏症中的神经溶酶体病理学表明鞘脂激活蛋白原具有重要的神经营养功能。
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Immunoreaction at 43 kDa with anti-ubiquitin antibody in breast neoplasms.乳腺肿瘤中与抗泛素抗体发生的43 kDa免疫反应。
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Misfolded major histocompatibility complex class I molecules accumulate in an expanded ER-Golgi intermediate compartment.错误折叠的主要组织相容性复合体I类分子在扩张的内质网-高尔基体中间腔室中积累。
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Suppressors of clathrin deficiency: overexpression of ubiquitin rescues lethal strains of clathrin-deficient Saccharomyces cerevisiae.网格蛋白缺乏的抑制因子：泛素的过表达挽救了网格蛋白缺陷型酿酒酵母的致死菌株。
Mol Cell Biol. 1993 Jan;13(1):521-32. doi: 10.1128/mcb.13.1.521-532.1993.
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Selective arrangement of ubiquitinated and D1 protein-containing nucleosomes within the Drosophila genome.果蝇基因组中泛素化和含D1蛋白核小体的选择性排列。
Cell. 1982 Feb;28(2):375-85. doi: 10.1016/0092-8674(82)90355-5.
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Metabolism of ubiquitinated histones.泛素化组蛋白的代谢
J Biol Chem. 1981 Jun 10;256(11):5916-20.
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Characterization of the heat-stable polypeptide of the ATP-dependent proteolytic system from reticulocytes.来自网织红细胞的ATP依赖性蛋白水解系统热稳定多肽的特性分析。
J Biol Chem. 1980 Aug 25;255(16):7525-8.
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Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections.胶体金颗粒在超薄冷冻组织切片双标记免疫电子显微镜中的应用。
J Cell Biol. 1981 Jun;89(3):653-65. doi: 10.1083/jcb.89.3.653.
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A new method of preparing gold probes for multiple-labeling cytochemistry.一种用于多标记细胞化学的金探针制备新方法。
Eur J Cell Biol. 1985 Jul;38(1):87-93.
9
Immunogold cytochemistry of cytochromes P-450 in porcine adrenal cortex. Two enzymes (side-chain cleavage and 11 beta-hydroxylase) are co-localized in the same mitochondria.
Histochemistry. 1987;86(6):551-7. doi: 10.1007/BF00489546.
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Specificity of binding of NH2-terminal residue of proteins to ubiquitin-protein ligase. Use of amino acid derivatives to characterize specific binding sites.蛋白质氨基末端残基与泛素-蛋白连接酶结合的特异性。使用氨基酸衍生物表征特异性结合位点。
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