具有细胞毒性的HLA - DQ3反应性人杂交瘤抗体4166，其可能区分DQ7 + 8与DQ9。

The cytotoxic HLA-DQ3 reactive human hybridoma antibody 4166 that may distinguish DQ7 + 8 from DQ9.

作者信息

Viken H D, Thoresen A B, Thorsby E, Hansen T

机构信息

Institute of Transplantation Immunology, National Hospital, Oslo, Norway.

出版信息

Hum Immunol. 1995 Apr;42(4):281-8. doi: 10.1016/0198-8859(94)00111-3.

DOI:10.1016/0198-8859(94)00111-3

PMID:7558911

Abstract

The human cytotoxic hybridoma antibody 4166 (IgM kappa) was generated by fusing an in vitro EBV-transformed B-LCL from a multiparous woman with the mouse-human heteromyeloma fusion partner CB-F7. In microcytotoxicity and IIF tests with B-LCLs as target cells, the mAb 4166 was specific for DQ3 (= DQ7 + 8 + 9). However, when used for DQ typing of class-II-positive PBMCs, 4166 could be rendered functionally specific for DQ7 + 8 and did not react with DQ9+ PBMCs. Binding of mAb 4166 to DQ8-positive cells was efficiently blocked by several allotype-specific mAbs recognizing DQ8. Other HLA class-II-specific mAbs were unable to inhibit. With the use of mAb 4166, it is possible to discriminate DQ7 + 8 from DQ9 in serologic DQ typing.

摘要

人细胞毒性杂交瘤抗体4166（IgM κ）是通过将一位经产妇的体外EBV转化的B淋巴细胞系与小鼠-人异源骨髓瘤融合伴侣CB-F7融合而产生的。在用B淋巴细胞系作为靶细胞的微量细胞毒性和间接免疫荧光试验中，单克隆抗体4166对DQ3（= DQ7 + 8 + 9）具有特异性。然而，当用于II类阳性外周血单核细胞（PBMC）的DQ分型时，4166在功能上可对DQ7 + 8具有特异性，且不与DQ9 + PBMC发生反应。几种识别DQ8的同种异型特异性单克隆抗体可有效阻断单克隆抗体4166与DQ8阳性细胞的结合。其他HLA II类特异性单克隆抗体则无法抑制。使用单克隆抗体4166，在血清学DQ分型中可以区分DQ7 + 8和DQ9。