Knezevic V, Spaventi R, Poljak L, Slade N, Svajger A, Pavelic K
Department of Molecular Medicine, Ruder Boskovic Institute, School of Medicine, University of Zagreb, Republic of Croatia.
J Anat. 1994 Aug;185 ( Pt 1)(Pt 1):181-7.
We have shown that the neu oncogene product (p185neu) is not present in the rat embryo before organogenesis. However, coincident with the onset of organogenesis, p185neu was detected in neural and connective tissue as well as in the secretory epithelium as was described by Kokai et al. (1987). In addition, p185neu is also expressed in the rat visceral yolk sac (VYS) endodermal cells but not in the mesenchymal and mesothelial layers of the same structure nor in the amnion. The first detectable sign of p185neu expression in VYS was found at d 11 of gestation and the levels of protein increased towards the end of pregnancy. In the yolk sac carcinoma (YSC), which is considered to be the malignant counterpart of the rat yolk sac, p185neu was observed only within columnar epithelial cells (the visceral component of the neoplasm) while parietal endoderm-like cells were devoid of detectable protein. From d 9 of pregnancy up to delivery some of the trophoblastic giant cells also showed a faint to moderate immunoreactivity. Results are presented which would indicate a possible role of p185neu in rat embryogenesis.
我们已经证明，神经癌基因产物（p185neu）在器官发生之前不存在于大鼠胚胎中。然而，与器官发生开始同时，如Kokai等人（1987年）所描述的，在神经和结缔组织以及分泌上皮中检测到了p185neu。此外，p185neu也在大鼠内脏卵黄囊（VYS）的内胚层细胞中表达，但在相同结构的间充质和间皮细胞层以及羊膜中不表达。在妊娠第11天发现VYS中p185neu表达的第一个可检测迹象，并且蛋白质水平在妊娠末期升高。在被认为是大鼠卵黄囊恶性对应物的卵黄囊癌（YSC）中，仅在柱状上皮细胞（肿瘤的内脏成分）中观察到p185neu，而壁层内胚层样细胞没有可检测到的蛋白质。从妊娠第9天到分娩，一些滋养层巨细胞也显示出微弱至中等的免疫反应性。给出的结果表明p185neu在大鼠胚胎发生中可能起作用。
