增加枯草芽孢杆菌gltA表达的GltC中的突变。
Mutations in GltC that increase Bacillus subtilis gltA expression.
作者信息
Belitsky B R, Sonenshein A L
机构信息
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
出版信息
J Bacteriol. 1995 Oct;177(19):5696-700. doi: 10.1128/jb.177.19.5696-5700.1995.
Abstract
Mutants with altered forms of GltC, a positive LysR-type regulator of Bacillus subtilis glutamate synthase gene expression, were isolated. The mutant GltC proteins stimulated expression from the wild-type gltA promoter region 1.5- to 2.0-fold and from mutant promoter regions up to 80-fold. Moreover, expression of gltA became much less dependent on a nitrogen source-associated signal.
摘要
分离出了枯草芽孢杆菌谷氨酸合酶基因表达的正向LysR型调节因子GltC形式发生改变的突变体。突变的GltC蛋白刺激野生型gltA启动子区域的表达提高了1.5至2.0倍，而刺激突变启动子区域的表达则提高了80倍。此外，gltA的表达对氮源相关信号的依赖性大大降低。
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