Funke G, Ramos C P, Collins M D
Department of Medical Microbiology, University of Zürich, Switzerland.
J Clin Microbiol. 1995 Aug;33(8):2091-7. doi: 10.1128/jcm.33.8.2091-2097.1995.
CDC coryneform group A-3 and A-4 bacteria were defined by Hollis and Weaver in 1981, but their taxonomic position is still unclear. By using biochemical and chemotaxonomical methods, four clinical strains belonging to CDC coryneform groups A-3 (n = 2) and A-4 (n = 2) were studied and could be assigned to the genus Cellulomonas, resulting in the first description of Cellulomonas strains isolated from clinical specimens. CDC coryneform group A-3 and A-4 strains were compared with the type strains of the seven species constituting the genus Cellulomonas at present as well as with the closely related species Oerskovia turbata, Oerskovia xanthineolytica, and Jonesia denitrificans, but their biochemical patterns were not compatible with the patterns of any of those species. Almost the entire sequences of the 16S rRNA genes of one representative strain of both CDC taxa were determined, and comparative sequence analysis confirmed the placement of the CDC coryneform group A-3 and A-4 strains studied in the Cellulomonas-Oerskovia subbranch of the actinomycetes. Both CDC taxa exhibited > 99% base pair homology within their 16S rDNAs. On the basis of phenotypic and molecular data, we formally propose a new species, Cellulomonas hominis sp. nov., for the CDC coryneform group A-3 bacteria examined. The type strain is DSM 9581. The precise taxonomic status of the CDC coryneform group A-4 strains studied remains to be established by quantitative DNA-DNA hybridizations.
1981年，霍利斯和韦弗定义了CDC棒状杆菌A-3群和A-4群细菌，但它们的分类地位仍不明确。通过生化和化学分类学方法，对属于CDC棒状杆菌A-3群（n = 2）和A-4群（n = 2）的4株临床菌株进行了研究，结果表明它们可归为纤维单胞菌属，这是首次对从临床标本中分离出的纤维单胞菌菌株进行描述。将CDC棒状杆菌A-3群和A-4群菌株与目前构成纤维单胞菌属的7个种的模式菌株以及密切相关的黄嘌呤奥氏杆菌、解黄嘌呤奥氏杆菌和反硝化琼斯氏菌进行了比较，但它们的生化模式与这些种中的任何一种都不相符。测定了两个CDC分类单元的一株代表性菌株的16S rRNA基因几乎全部序列，比较序列分析证实了所研究的CDC棒状杆菌A-3群和A-4群菌株在放线菌的纤维单胞菌-奥氏杆菌亚分支中的位置。两个CDC分类单元在其16S rDNA内表现出> 99%的碱基对同源性。基于表型和分子数据，我们正式为所检测的CDC棒状杆菌A-3群细菌提出一个新种，即人纤维单胞菌（Cellulomonas hominis sp. nov.）。模式菌株为DSM 9581。所研究的CDC棒状杆菌A-4群菌株的确切分类地位仍有待通过定量DNA-DNA杂交来确定。
