牛病毒性腹泻病毒的一株野毒株与疫苗株之间的抗原差异
Antigenic differences between a field isolate and vaccine strains of bovine viral diarrhea virus.
作者信息
Reddy J R, Xue W, Rivera S, Minocha H C
机构信息
Department of Diagnostic Medicine/Pathology, Kansas State University, Manhattan 66506, USA.
出版信息
J Clin Microbiol. 1995 Aug;33(8):2159-61. doi: 10.1128/jcm.33.8.2159-2161.1995.
Antigenic diversity among the bovine viral diarrhea virus (BVDV) cytopathic strains 87-2552 (field isolate) and NADL and Singer (prototype strains) was demonstrated with monoclonal antibodies (MAbs) in enzyme-linked immunosorbent, immunofluorescence, virus neutralization, and immunoprecipitation assays. Two MAbs against BVDV strain 87-2552, designated D11 and B7, strongly neutralized this field strain and were specific for the 48-kDa glycoprotein of the virus. These two MAbs have different subisotypes, immunoglobulin G1 for D11 and immunoglobulin G3 for B7. MAbs against BVDV strains 87-2552 and NADL were specific for their respective strains in virus neutralization assays. The results indicated significant antigenic differences between BVDV strain 87-2552 and the NADL and Singer strains.
在酶联免疫吸附试验、免疫荧光试验、病毒中和试验及免疫沉淀试验中，利用单克隆抗体（MAb）证明了牛病毒性腹泻病毒（BVDV）细胞病变株87 - 2552（野毒株）与NADL株和辛格株（原型株）之间的抗原多样性。两种针对BVDV株87 - 2552的单克隆抗体，命名为D11和B7，能强烈中和该野毒株，且对病毒的48 kDa糖蛋白具有特异性。这两种单克隆抗体具有不同的亚类，D11为免疫球蛋白G1，B7为免疫球蛋白G3。在病毒中和试验中，针对BVDV株87 - 2552和NADL株的单克隆抗体对各自的毒株具有特异性。结果表明，BVDV株87 - 2552与NADL株和辛格株之间存在显著的抗原差异。
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