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骆驼抗A型和牛病毒性腹泻病毒联合疫苗的制备及田间研究

Preparation and field study of combined vaccine against type A and bovine viral diarrhea virus in camels.

作者信息

Elhelw Hamed Adel, El Fadeel Maha Raafat Abd, El-Sergany Elham, Allam Ahmad, Elbayoumy Mohamed Karam, El-Kattan Adel Mahrous, El-Kholy Alaa Abdel-Moneim

机构信息

Veterinary Serum and Vaccine Research Institute, Cairo, Egypt.

Department of Rinder Pest like Diseases, Veterinary Serum and Vaccine Research Institute, Agriculture Research Center, Cairo, Egypt.

出版信息

Clin Exp Vaccine Res. 2022 Jan;11(1):30-42. doi: 10.7774/cevr.2022.11.1.30. Epub 2022 Jan 31.

DOI:10.7774/cevr.2022.11.1.30
PMID:35223663
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8844669/
Abstract

PURPOSE

The key objective of this study was to formulate a local combined inactivated gel adjuvanted vaccine containing bovine viral diarrhea virus (BVDV)-1, BVDV-2 viruses and type A toxoid. The study evaluated its ability to enhance protective active immune response in camels' calves against these infectious pathogens under field conditions.

MATERIALS AND METHODS

The local BVDV cytopathic strains and a local strain of toxigenic type A were used in vaccines formulation. Vaccines A and B were monovalent vaccines against and both strains of BVDVs, respectively. While the vaccine C was the combined vaccine used against the three agents. All vaccines were adjuvanted with Montanide gel. Sterility, safety, and potency tests were applied on the formulated vaccines. Virus neutralization and toxin anti-toxin neutralization tests were used to evaluate the immune responses.

RESULTS

Both monovalent (vaccine A) and combined vaccines (vaccine C) showed a protective level (4.5 and 3 IU/mL, respectively) against from the 2nd-week post-vaccination. The titer declined to 3 and 2 IU/mL, respectively at the 5th-month post-vaccination. The titer against BVDV, the monovalent vaccine (vaccine B) reached the beak (1.95 IU/mL) at the 1st-month post-vaccination and lasted till 6th-month post-vaccination (0.92 and 0.94 IU/mL) for BVDV-1a and BVDV-2, respectively.

CONCLUSION

Vaccination of camels with the combined vaccine adjuvanted by Montanide gel containing type A toxoid and BVDV strains with 6-month intervals is recommended to protect camels safely and efficiently against such infections in the field.

摘要

目的

本研究的主要目标是制备一种含有牛病毒性腹泻病毒（BVDV）-1、BVDV-2病毒和A型类毒素的局部联合灭活凝胶佐剂疫苗。该研究评估了其在野外条件下增强骆驼幼崽针对这些传染性病原体的保护性主动免疫反应的能力。

材料与方法

使用当地的BVDV细胞病变株和当地的产毒A型菌株制备疫苗。疫苗A和疫苗B分别是针对两种BVDV菌株的单价疫苗。而疫苗C是用于针对三种病原体的联合疫苗。所有疫苗均用Montanide凝胶佐剂。对制备的疫苗进行无菌、安全性和效力测试。采用病毒中和试验和毒素抗毒素中和试验评估免疫反应。

结果

单价疫苗（疫苗A）和联合疫苗（疫苗C）在接种后第2周均显示出针对[此处原文缺失相关内容]的保护水平（分别为4.5和3 IU/mL）。接种后第5个月，效价分别降至3和2 IU/mL。针对BVDV的单价疫苗（疫苗B）在接种后第1个月达到峰值（1.95 IU/mL），对于BVDV-1a和BVDV-2，分别持续到接种后第6个月（0.92和0.94 IU/mL）。

结论

建议每隔6个月用含有A型类毒素和BVDV菌株的Montanide凝胶佐剂联合疫苗对骆驼进行接种，以在野外安全有效地保护骆驼免受此类感染。

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