囊性纤维化患者铜绿假单胞菌分离株的核糖体分型与基因组指纹图谱比较
Comparison of ribotyping and genome fingerprinting of Pseudomonas aeruginosa isolates from cystic fibrosis patients.
作者信息
Bennekov T, Colding H, Ojeniyi B, Bentzon M W, Høiby N
机构信息
Institute of Medical Microbiology and Immunology, University of Copenhagen, Denmark.
出版信息
J Clin Microbiol. 1996 Jan;34(1):202-4. doi: 10.1128/jcm.34.1.202-204.1996.
Abstract
Forty Pseudomonas aeruginosa strains, previously characterized by pulsed-field gel electrophoresis, were ribotyped with EcoRI, BamHI, ClaI, and PvuII. Ribotyping with PvuII proved to be as discriminatory as pulsed-field gel electrophoresis with XbaI or DraI while EcoRI and BamHI were not. ClaI contributed further ribotypes, some of which might be due to a transposable element.
摘要
四十株先前通过脉冲场凝胶电泳进行特征分析的铜绿假单胞菌菌株，用EcoRI、BamHI、ClaI和PvuII进行核糖分型。结果表明，用PvuII进行核糖分型的鉴别能力与用XbaI或DraI进行脉冲场凝胶电泳的鉴别能力相当，而EcoRI和BamHI则不然。ClaI产生了更多的核糖型，其中一些可能归因于转座元件。
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