Burks A W, Cockrell G, Stanley J S, Helm R M, Bannon G A
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
J Clin Invest. 1995 Oct;96(4):1715-21. doi: 10.1172/JCI118216.
Peanut allergy is a significant health problem because of the frequency, the potential severity, and the chronicity of the allergic sensitivity. Serum IgE from patients with documented peanut hypersensitivity reactions and a peanut cDNA expression library were used to identify clones that encode peanut allergens. One of the major peanut allergens, Ara h I, was selected from these clones using Ara h I specific oligonucleotides and polymerase chain reaction technology. The Ara h I clone identified a 2.3-kb mRNA species on a Northern blot containing peanut poly (A)+ RNA. DNA sequence analysis of the cloned inserts revealed that the Ara h I allergen has significant homology with the vicilin seed storage protein family found in most higher plants. The isolation of the Ara h I clones allowed the synthesis of this protein in E. coli cells and subsequent recognition of this recombinant protein in immunoblot analysis using serum IgE from patients with peanut hypersensitivity. With the production of the recombinant peanut protein it will now be possible to address the pathophysiologic and immunologic mechanisms regarding peanut hypersensitivity reactions specifically and food hypersensitivity in general
由于过敏敏感性的发生频率、潜在严重性和慢性，花生过敏是一个重大的健康问题。来自有记录的花生过敏反应患者的血清IgE和花生cDNA表达文库被用于鉴定编码花生过敏原的克隆。使用Ara h I特异性寡核苷酸和聚合酶链反应技术从这些克隆中筛选出一种主要的花生过敏原Ara h I。Ara h I克隆在含有花生聚腺苷酸加尾RNA的Northern印迹上鉴定出一个2.3 kb的mRNA种类。对克隆插入片段的DNA序列分析表明，Ara h I过敏原与大多数高等植物中发现的豌豆球蛋白种子贮藏蛋白家族具有显著同源性。Ara h I克隆的分离使得该蛋白能够在大肠杆菌细胞中合成，并随后在使用花生过敏患者血清IgE的免疫印迹分析中识别这种重组蛋白。随着重组花生蛋白的产生，现在有可能专门研究花生过敏反应以及一般食物过敏的病理生理和免疫机制。
