Rihs H P, Rozynek P, May-Taube K, Welticke B, Baur X
Abteilung Molekulare Genetik, Berufsgenossenschaftliches, Forschungsinstitut für Arbeitsmedizin, Ruhr-Universität, Bochum, Deutschland.
Int Arch Allergy Immunol. 1994 Oct;105(2):190-4. doi: 10.1159/000236824.
Highly degenerate profilin-specific primers were used to amplify by polymerase chain reaction the profilin-coding sequence of wheat cDNA. Analyzing the sequencing data after subcloning revealed the existence of three isoforms. The high amino acid sequence identity with the birch pollen allergen Betv II suggests that wheat profilin may be a potential plant allergen, relevant in food allergy.
使用高度简并的肌动蛋白结合蛋白特异性引物，通过聚合酶链反应扩增小麦cDNA的肌动蛋白结合蛋白编码序列。亚克隆后分析测序数据，发现存在三种同工型。与桦树花粉过敏原Betv II的高氨基酸序列同一性表明，小麦肌动蛋白结合蛋白可能是一种潜在的植物过敏原，与食物过敏有关。
