评估Uricult-Trio中一种新型琼脂用于快速检测尿液中大肠杆菌的效果。
Evaluation of a new agar in Uricult-Trio for rapid detection of Escherichia coli in urine.
作者信息
Dalet F, Segovia T
机构信息
Microbiology Department, Puigvert Foundation, Barcelona, Spain.
出版信息
J Clin Microbiol. 1995 May;33(5):1395-8. doi: 10.1128/jcm.33.5.1395-1398.1995.
Abstract
A new commercial agar (Uricult-Trio) with 8-hydroxyquinoline-beta-glucuronide was used to assess 2,536 uropathogens for beta-glucuronidase activity typical of Escherichia coli. Included in the study were 1,807 strains of the family Enterobacteriaceae, 284 strains of nonfermentative bacilli, 345 strains of gram-positive cocci, and 100 yeast strains. In identifying E. coli, the test agar gave a sensitivity of 95.5% and a specificity of 97.2%. Fifty E. coli isolates gave negative reactions; 31 non-E. coli strains produced black colonies characteristic of E. coli. No growth of gram-positive cocci and no false-positive reactions from yeasts were observed. The recovery rate for E. coli on this agar was at least 10% higher than that on blood agar.
摘要
一种含有8-羟基喹啉-β-葡萄糖醛酸的新型商业琼脂（Uricult-Trio）被用于评估2536株尿路病原体的β-葡萄糖醛酸酶活性，这种活性是大肠杆菌所特有的。该研究包括1807株肠杆菌科菌株、284株非发酵杆菌、345株革兰氏阳性球菌和100株酵母菌株。在鉴定大肠杆菌时，测试琼脂的敏感性为95.5%，特异性为97.2%。50株大肠杆菌分离株呈阴性反应；31株非大肠杆菌菌株产生了大肠杆菌特有的黑色菌落。未观察到革兰氏阳性球菌生长，酵母也未出现假阳性反应。这种琼脂上大肠杆菌的回收率比血琼脂上至少高10%。
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