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本文引用的文献

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Detection of beta-glucuronidase in lactose-fermenting members of the family Enterobacteriaceae and its presence in bacterial urine cultures.肠杆菌科乳糖发酵菌中β-葡萄糖醛酸酶的检测及其在细菌尿培养物中的存在情况。
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Rapid and economical identification and antimicrobial susceptibility test methodology for urinary tract pathogens.用于尿路病原体的快速且经济的鉴定及抗菌药敏试验方法
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Detection of specific bacterial enzymes by high contrast metal chelate formation. Part II. Specific detection of Escherichia coli on multipoint-inoculated plates using 8-hydroxyquinoline-beta-D-glucuronide.
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Jan;267(3):316-21. doi: 10.1016/s0176-6724(88)80047-6.
4
Beta-glucuronidase (PGUA) and beta-xylosidase (ONPX) disc tests for rapid identification of urinary coliforms.用于快速鉴定尿中大肠菌的β-葡萄糖醛酸酶（PGUA）和β-木糖苷酶（ONPX）纸片试验。
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Rapid identification of Escherichia coli by Fluorocult media and positive indole reaction.通过荧光培养基和阳性吲哚反应快速鉴定大肠杆菌。
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Rapid detection of Escherichia coli in urine samples by a new chromogenic beta-glucuronidase assay.
J Clin Microbiol. 1989 Apr;27(4):778-9. doi: 10.1128/jcm.27.4.778-779.1989.
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[The rapid identification of E. coli by the detection of 3 enzymes].[通过检测三种酶快速鉴定大肠杆菌]
Berl Munch Tierarztl Wochenschr. 1989 Mar 1;102(3):93-5.
8
Effect of calcium glucarate on beta-glucuronidase activity and glucarate content of certain vegetables and fruits.葡萄糖醛酸钙对某些蔬菜和水果中β-葡萄糖醛酸酶活性及葡萄糖醛酸含量的影响。
Biochem Med Metab Biol. 1990 Apr;43(2):83-92. doi: 10.1016/0885-4505(90)90012-p.
9
Comparison of two test kits for rapid identification of Escherichia coli by a beta-glucuronidase assay.
Eur J Clin Microbiol Infect Dis. 1990 Feb;9(2):151-2. doi: 10.1007/BF01963647.
10
Rapid detection of urinary tract infection caused by Escherichia coli or Proteeae species.快速检测由大肠杆菌或变形杆菌属引起的尿路感染。
Eur J Clin Microbiol Infect Dis. 1991 Nov;10(11):922-6. doi: 10.1007/BF02005445.

评估Uricult-Trio中一种新型琼脂用于快速检测尿液中大肠杆菌的效果。

Evaluation of a new agar in Uricult-Trio for rapid detection of Escherichia coli in urine.

作者信息

Dalet F, Segovia T

机构信息

Microbiology Department, Puigvert Foundation, Barcelona, Spain.

出版信息

J Clin Microbiol. 1995 May;33(5):1395-8. doi: 10.1128/jcm.33.5.1395-1398.1995.

DOI:10.1128/jcm.33.5.1395-1398.1995
PMID:7615766
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228177/
Abstract

A new commercial agar (Uricult-Trio) with 8-hydroxyquinoline-beta-glucuronide was used to assess 2,536 uropathogens for beta-glucuronidase activity typical of Escherichia coli. Included in the study were 1,807 strains of the family Enterobacteriaceae, 284 strains of nonfermentative bacilli, 345 strains of gram-positive cocci, and 100 yeast strains. In identifying E. coli, the test agar gave a sensitivity of 95.5% and a specificity of 97.2%. Fifty E. coli isolates gave negative reactions; 31 non-E. coli strains produced black colonies characteristic of E. coli. No growth of gram-positive cocci and no false-positive reactions from yeasts were observed. The recovery rate for E. coli on this agar was at least 10% higher than that on blood agar.

摘要

一种含有8-羟基喹啉-β-葡萄糖醛酸的新型商业琼脂（Uricult-Trio）被用于评估2536株尿路病原体的β-葡萄糖醛酸酶活性，这种活性是大肠杆菌所特有的。该研究包括1807株肠杆菌科菌株、284株非发酵杆菌、345株革兰氏阳性球菌和100株酵母菌株。在鉴定大肠杆菌时，测试琼脂的敏感性为95.5%，特异性为97.2%。50株大肠杆菌分离株呈阴性反应；31株非大肠杆菌菌株产生了大肠杆菌特有的黑色菌落。未观察到革兰氏阳性球菌生长，酵母也未出现假阳性反应。这种琼脂上大肠杆菌的回收率比血琼脂上至少高10%。