Horibe M, Watanabe H, Ishikawa I
Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
J Clin Periodontol. 1995 Jul;22(7):510-5. doi: 10.1111/j.1600-051x.1995.tb00798.x.
Serum IgG antibody titers to 7 periodontopathic bacteria in periodontitis patients were measured at the 1st visit and after various periodontal treatments with clinically successful improvement, in order to evaluate what kind of factors are associated with changes of serum antibody titers. 20 patients (10 male and 10 female from 23 to 61 years old) with adult, rapidly progressive periodontitis were enrolled in this study. All patients received initial preparation and most of them also underwent surgical procedure. After the treatments, the mean probing pocket depths decreased from 3.72 mm to 1.56 mm. Serum samples were collected from patients at the initial and final examinations. Serum IgG antibody titers against sonicated antigens of Porphyromonas gingivalis FDC 381, Prevotella intermedia ATCC 25611, Prevotella loescheii ATCC 15930, Fusobacterium nucleatum subspecies nucleatum ATCC 25586, Actinobacillus actinomycetemcomitans FDC Y4, Eikenella corrodens FDC 1073 and Capnocytophaga ochracea # M 12 were determined by enzyme-linked immunosorbent assay. The mean antibody titers to P. gingivalis and P. intermedia decreased significantly after the treatment as compared to their pretreatment levels. The antibody titer to P. gingivalis, especially, decreased in all of the patients examined. A significant relationship was found between the decreased antibody titer to P. gingivalis and the number of teeth which received periodontal surgery, as well as treatment length, and the relationship between the decreased antibody titer to P. intermedia and the number of extracted teeth was also significant. These results suggest that the changes of serum IgG titers against P. gingivalis and P. intermedia are related to the suppression of such pathogens in subgingival plague.
为了评估哪些因素与血清抗体滴度的变化相关，在首次就诊时以及经过各种牙周治疗且临床症状成功改善后，对牙周炎患者血清中针对7种牙周病原菌的IgG抗体滴度进行了测量。本研究纳入了20例（年龄在23至61岁之间，男性10例，女性10例）患有成人快速进展性牙周炎的患者。所有患者均接受了初始准备，大多数患者还接受了外科手术。治疗后，平均探诊深度从3.72毫米降至1.56毫米。在初始和最终检查时采集患者的血清样本。采用酶联免疫吸附测定法测定血清中针对牙龈卟啉单胞菌FDC 381、中间普氏菌ATCC 25611、洛氏普氏菌ATCC 15930、具核梭杆菌具核亚种ATCC 25586、伴放线放线杆菌FDC Y4、腐蚀艾肯菌FDC 1073和黄褐二氧化碳嗜纤维菌# M 12超声处理抗原的IgG抗体滴度。与治疗前水平相比，治疗后针对牙龈卟啉单胞菌和中间普氏菌的平均抗体滴度显著降低。尤其是针对牙龈卟啉单胞菌的抗体滴度在所有检查患者中均降低。发现针对牙龈卟啉单胞菌的抗体滴度降低与接受牙周手术的牙齿数量以及治疗时长之间存在显著关系，针对中间普氏菌的抗体滴度降低与拔牙数量之间的关系也很显著。这些结果表明，血清中针对牙龈卟啉单胞菌和中间普氏菌的IgG滴度变化与龈下菌斑中此类病原菌的抑制有关。
